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Vesicular transfer of membrane components to bovine epididymal spermatozoa

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Abstract

Epididymosomes (apocrine secreted epididymal vesicles) are assumed to play a crucial role in sperm maturation. Our aim has been to analyze the fusogenic properties of bovine epididymosomes and their involvement in the transfer of membrane components (lipids, proteins, plasma membrane Ca2+-ATPase 4 [PMCA4]) into bovine sperm. The fusogenic properties of epididymosomes with spermatozoa were investigated in vitro by using octadecyl rhodamine-B (R18)-labeled epididymosomes. Spermatozoa isolated from the epididymal caput showed a higher fusion rate than those taken from the cauda. The fusion rate was dependent on pH and time. Furthermore, the lipid and protein content in spermatozoa changed during epididymal transit and after in vitro fusion with epididymosomes. Following the in vitro fusion of caput spermatozoa with epididymosomes, the cholesterol/total phospholipid ratio of the sperm plasma membrane decreased. The effect was comparable with the cholesterol/total phospholipid ratio of native cauda spermatozoa. Co-incubation experiments of spermatozoa with biotinylated epididymosomes additionally revealed that proteins were transferred from epididymosomes to sperm. To examine the potential transfer of epididymis-derived PMCA4 to spermatozoa, immunofluorescence analysis and Ca2+-ATPase activity assays were performed. In caput spermatozoa, the PMCA4 fluorescence signal was slightly raised and Ca2+-ATPase activity increased after in vitro fusion. Thus, our experiments indicate significant changes in the lipid and protein composition of epididymal sperm following interaction with epididymosomes. Moreover, our results substantiate the presumption that PMCA4 is transferred to spermatozoa via epididymosomes.

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Acknowledgments

The authors are greatly indebted to Anne Henkeler, Gudrun Hoffbauer and Claudia Keppler for their expert technical help.

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Authors and Affiliations

  1. Department of Anatomy and Cell Biology, Philipps University of Marburg, Robert-Koch-Strasse 8, 35037, Marburg, Germany

    A. Schwarz, H. Post, T. Brandenburger, G. Aumüller & B. Wilhelm

  2. Department of Anatomy, University Clinic Essen, Essen, Germany

    G. Wennemuth

  3. Department of Anesthesiology, University Hospital Düsseldorf, Düsseldorf, Germany

    T. Brandenburger

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  1. A. Schwarz
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  2. G. Wennemuth
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  6. B. Wilhelm
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Correspondence to B. Wilhelm.

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Schwarz, A., Wennemuth, G., Post, H. et al. Vesicular transfer of membrane components to bovine epididymal spermatozoa. Cell Tissue Res 353, 549–561 (2013). https://doi.org/10.1007/s00441-013-1633-7

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