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Measurement of oxidative stress in the follicular fluid of infertility patients with an endometrioma

  • Gynecologic Endocrinology and Reproductive Medicine
  • Published:
Archives of Gynecology and Obstetrics Aims and scope Submit manuscript

Abstract

Purpose

Follicular fluid (FF) might reflect the environment during follicle and oocyte growth, and an evaluation of oxidative stress in the FF might be useful in predicting oocyte quality. In order to measure the oxidative stress (OS) in the FF from a single follicle of patients with endometrioma (EM), we evaluated whether an EM might affect the environment of follicular growth.

Methods

Between December 2011 and July 2013, 26 patients with a unilateral EM (EM group) and 29 without EM (control group) were enrolled in this study. The FF was obtained during the first puncture of follicular aspiration, and was stored at −30 °C until it was assayed. A Free Radical Elective Evaluator (WISMERLL, USA) was used to perform d-ROM and BAP tests to measure oxidative stress (U.CARR) and antioxidant power (μmol/L).

Results

The d-ROM values in the EMC and control groups were 328.7 ± 97.8 and 414.9 ± 84.2, respectively, and the BAP values for the two groups were 2474.3 ± 432.0 and 2552.8 ± 435.58, respectively. These values were similar between the two groups (mean ± SD). The number of patients with a modified BAP/d-ROM ratio of <1.0 in the EM group was similar to that for the control group at 16 and 15, respectively (61.5 and 51.7 %).

Conclusions

The oxidative stress and antioxidant potential in the FF of the patients with unilateral EM showed values similar to those without an EM. Therefore, we concluded that EMs do not affect the environment for follicle growth during ART treatment.

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References

  1. Nakagawa K, Ohgi S, Kojima R, Itoh M, Horikawa T, Irahara M, Saito H (2006) Reduction of perifollicular arterial blood flow resistance after hCG administration is a good indicator of the recovery of mature oocytes in ART treatment. J Assist Reprod Genet 23:433–438

    Article  PubMed  PubMed Central  Google Scholar 

  2. Harada T, Iwabe T, Terakawa N (2001) Role of cytokines in endometriosis. Fertil Steril 76:1–10

    Article  PubMed  CAS  Google Scholar 

  3. Sanchez AM, Papaleo E, Corti L, Santambrogio P, Levi S, Viganò P, Candiani M, Panina-Bordignon P (2014) Iron availability is increased in individual human ovarian follicles in close proximity to an endometrioma compared with distal ones. Hum Reprod 29:577–583

    Article  PubMed  CAS  Google Scholar 

  4. Hracsko Z, Safar Z, Orvos H, Ovak Z, Pal A, Varga IS (2007) Evaluation of oxidative stress markers after vaginal delivery or Cesarean section. In Vivi 21:703–706

    CAS  Google Scholar 

  5. Franco MC, Kawamoto EM, Gorjão R, Rastelli VM, Curi R, Scavone C, Sawaya AL, Fortes ZB, Sesso R (2007) Biomarkers of oxidative stress and antioxidant status in children born small for gestational age: evidence of lipid peroxidation. Pediatr Res 62:204–208

    Article  PubMed  CAS  Google Scholar 

  6. Brakke K, Bechensteen AG, Halvorsen BL, Blomhoff R, Haaland K, Staff AC (2007) Oxidative stress markers and antioxidant status after oral iron supplementation to very low birth weight infants. J Pediatr 151:23–28

    Article  Google Scholar 

  7. Hussein MH, Daoud GA, Kakita H, Hattori A, Murai H, Yasuda M, Mizuno K, Goto K, Ozaki Y, Ito T, Tanaka T, Fukuda S, Kato I, Fujimoto S, Suzuki S, Sobajima H, Togari H (2007) The sex differences of cerebrospinal fluid levels of interleukin 8 and antioxidants in asphyxiated newborn. Shock 28:154–159

    Article  PubMed  CAS  Google Scholar 

  8. Nakagawa K, Nishi Y, Sugiyama R, Jyuen H, Kuribayashi Y, Sugiyama R, Inoue M (2012) A programmed schedule of oocyte retrieval using mild ovarian stimulation (clomiphene citrate and recombinant follicular stimulating hormone). Reprod Med Biol 11:85–89

    Article  CAS  Google Scholar 

  9. Buonocore G, Perrone S, Longini M, Terzuoli L, Bracci R (2000) Total hydroperoxide and advanced oxidation proteins products in preterm hypoxic babies. Pediatr Res 47:221–224

    Article  PubMed  CAS  Google Scholar 

  10. Fukui T, Yamauchi K, Maruyama M, Yasuda T, Kohno M, Abe Y (2011) Significance of measuring oxidative stress in lifestyle-related diseases from the viewpoint of correlation between d-ROMs and BAP in Japanese subjects. Hypertens Res 14:1041–1045

    Article  Google Scholar 

  11. Pasquini A, Luchetti E, Marchetti V, Cardini G, Iorio EL (2008) Analytical performances of d-ROMs test and BAP test in canine plasma: definition of the normal range in healthy labrador dogs. Vet Res Commun 14:137–143

    Article  Google Scholar 

  12. Dohi K, Satoh K, Ohtaki H, Shioda S, Miyake Y, Shindo M, Aruga T (2005) Elevated plasma levels of bilirubin in patients with neurotrauma reflect its pathophysiological role in free radical scavenging. In Vivo 19:855–860

    PubMed  CAS  Google Scholar 

  13. Cornelli U, Terranova R, Luca S, Cornelli M, Alberti A (2001) Bioavailability and antioxidant activity of some food supplements in men and women using the D-Roms test as a marker of oxidative stress. J Nutr 14:3208–3211

    Google Scholar 

  14. Tarín JJ, Vendrell FJ, Ten J, Cano A (1996) The oxidizing agent tertiary butyl hydroperoxide induces disturbances in spindle organization, c-meiosis, and aneuploidy in mouse oocytes. Mol Hum Reprod 2:895–901

    Article  PubMed  Google Scholar 

  15. Zhang X, Wu XQ, Lu S, Guo YL, Ma X (2006) Deficit of mitochondria-derived ATP during oxidative stress impairs mouse MII oocyte spindle. Cell Res 16:841–850

    Article  PubMed  CAS  Google Scholar 

  16. Barroso G, Barrionuevo M, Rao P, Graham L, Danforth D, Huey S et al (1999) Vascular endothelial growth factor, nitric oxide and leptin follicular fluid levels correlate negatively with embryo quality in IVF patients. Fertil Steril 72:1024–1026

    Article  PubMed  CAS  Google Scholar 

  17. Mendoza C, Ruiz-Requena E, Ortega E, Cremades N, Martinez F, Bernabeu R, Greco E, Tesarik J (2002) Follicular fluid markers of oocyte developmental potential. Hum Reprod 17:1017–1022

    Article  PubMed  CAS  Google Scholar 

  18. Arnot AM, Vandekerckhove P, DeBono MA, Rutherford AJ (1995) Follicular volume and number during in vitro fertilization: association with oocyte developmental capacity and pregnancy rate. Hum Reprod 10:256–261

    PubMed  CAS  Google Scholar 

  19. Pasqualotto EB, Agarwal A, Sharma RK, Izzo VM, Pinotti JA, Joshi NJ, Rose BI (2004) Effect of oxidative stress in follicular fluid on the outcome of assisted reproductive procedure. Fertil Steril 81:973–976

    Article  PubMed  CAS  Google Scholar 

  20. Sabatini L, Wilson C, Lower A, Al-Shawaf T, Grudzinskas JG (1999) Superoxide dismutase activity in human follicular fluid after controlled ovarian hyperstimulation in women undergoing in vitro fertilization. Fertil Steril 72:1027–1034

    Article  PubMed  CAS  Google Scholar 

  21. Wiener-Megnazi Z, Vardi L, Lissak A, Shnizer S, Reznick AZ, Ishai D, Lahav-Baratz S, Shiloh H, Koifman M, Dirnfeld M (2004) Oxidative stress induces in follicular fluid as measured by the thermochemiluminescence assay correlate with outcome parameters in in vitro fertilization. Fertil Steril 82(Suppl 3):1171–1176

    Article  PubMed  CAS  Google Scholar 

  22. Werbrouck E, Spiessens C, Meuleman C, D’Hooghe T (2006) No difference in cycle pregnancy rate and in cumulative love-birth rate between women with surgically treated minimal to mild endometriosis and women with unexplained infertility after controlled ovarian hyperstimulation and intrauterine insemination. Fertil Steril 86:566–571

    Article  PubMed  Google Scholar 

  23. Opoien HK, Fedorcsak P, Byholm T, Tanbo T (2011) Complete surgical removal of minimal or mild endometriosis improves outcome of subsequent IVF/ICSI treatment. Reprod Biomed Online 23:389–395

    Article  PubMed  CAS  Google Scholar 

  24. Opøien HK, Fedorcsak P, Polec A, Stensen MH, Åbyhol T, Tanbo T (2013) Do endometriomas induce an inflammatory reaction in nearby follicles? Hum Reprod 28:1837–1845

    Article  PubMed  Google Scholar 

  25. Sugiyama R, Nakagawa K, Hisano M, Mitui M, Yamaguchi K (2014) The ovarian stimulation method can cause oxidative stress in the follicular fluid. Hum Reprod 29(supp 1):i309–1310 (P-463)

    Google Scholar 

  26. Yamaguchi K, Mandai M, Toyokuni S, Hamanishi J, Higuchi T, Takakura K, Fujii S (2008) Contents of endometriotic cysts, especially the high concentration of free iron, are a possible cause of carcinogenesis in the cysts through the iron-induced persistent oxidative stress. Clin Cancer Res 14:32–40

    Article  PubMed  CAS  Google Scholar 

  27. Prieto L, Quesada JF, Cambero O, Pacheco A, PellicerA Codoceo R, Garcia-Velasco JA (2012) Analysis of follicular fluid and serum markers of oxidative stress in women with infertility related to endometriosis. Fertil Steril 98:126–130

    Article  PubMed  CAS  Google Scholar 

  28. Coccia ME, Rizzello F, Barone S, Pinelli S, Rapalini E, Parri C, Caracciolo D, Papageorgiou S, Cima G, Gandini L (2014) Is there a critical endometrioma size associated with reduced ovarian responsiveness in assisted reproduction techniques? Reprod Biomed Online 29:259–266

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Division of Reproductive Medicine, Sugiyama Clinic, 1-53-1, Ohara, Setagaya, Tokyo, 156-0041, Japan

    Koji Nakagawa & Rikikazu Sugiyama

  2. Department of Maternal-Fetal Biology, National Center for Child Health and Development, Tokyo, Japan

    Michi Hisano & Koushi Yamaguchi

Authors
  1. Koji Nakagawa
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  2. Michi Hisano
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  4. Koushi Yamaguchi
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Correspondence to Koji Nakagawa.

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The authors have received no funding for this study, and they have no financial interest in any companies.

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Nakagawa, K., Hisano, M., Sugiyama, R. et al. Measurement of oxidative stress in the follicular fluid of infertility patients with an endometrioma. Arch Gynecol Obstet 293, 197–202 (2016). https://doi.org/10.1007/s00404-015-3834-7

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  • DOI: https://doi.org/10.1007/s00404-015-3834-7

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