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Heat shock protein 27 protects the heart against myocardial infarction

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Abstracts.

Heat shock proteins (hsp) represent a group of chaperones which protects the cells against a diversity of stresses. It has been demonstrated that hsp27 is constitutively present in cells where it plays an important role in different cytoprotective processes which ultimately inhibit cell death. We investigated the response of the isolated perfused mouse heart over expressing hsp27 to the ischaemia/reperfusion injury using infarct size as an end point. Our results show for the first time that mice over expressing hsp27 (verified by Western blotting analysis) were found to be protected from lethal ischaemia/reperfusion injury compared to their negative littermates.

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References

  1. Akbar MT, Lundberg AMC, Liu K, Vidyadaran S, Wells KE, Dolatshad H, Wynn S, Wells DJ, Latchman DS, de Belleroche J (2003) The neuroprotective effects of heat shock protein 27 overexpression in transgenic animals against Kainate-induced seizures and hippocampal cell death. Journal of Biological Chemistry 278:19956–19965

    Article  CAS  PubMed  Google Scholar 

  2. Bell RM, Yellon DM (2003) Atorvastatin, administered at the onset of reperfusion, and independent of lipid lowering, protects the myocardium by up-regulating a pro-survival pathway. J Am Coll Cardiol 41:508–515

    Article  CAS  PubMed  Google Scholar 

  3. Brar BS, Stephanou AS, Wagstaff MJD, Coffin RS, Marber MS, Engelman G, Latchman DS (1999) Heat shock proteins delivered with a virus vector can protect cardiac cells against apoptosis as well as against thermal or ischaemic stress. Journal of Molecular and Cellular Cardiology 31:135–146

    Article  CAS  PubMed  Google Scholar 

  4. Chi NC, Karliner JS (2004) Molecular determinants of responses to myocardial ischaemia/reperfusion injury: focus on hypoxya-inducible and heat shock factors. Cardiovasc Res 61:437–447

    Article  CAS  PubMed  Google Scholar 

  5. Heads RJ, Latchman DS, Yellon DM (1994) Stable high level expression of a transfected human HSP70 gene protects a heart-derived muscle cell line against thermal stress. Journal of Molecular and Cellular Cardiology 26:695–699

    Article  CAS  PubMed  Google Scholar 

  6. Heads RJ, Yellon DM, Latchman DS (1995) Differential cytoprotection against heat stress or hypoxia following expression of specific stress protein genes in myogenic cells. Journal of Molecular and Cellular Cardiology 27:1669–1678

    Article  CAS  PubMed  Google Scholar 

  7. Hollander JM, Martin JL, Belke DD, Scott BT, Dillmann WH (2003) Transgenic overexpression of wild type hsp27 and a hsp27 nonphosphorylatable mutant protects against ischaemia/reperfusion injury. Circulation (supplement) 108:abst 431

    Google Scholar 

  8. Kalwy S, Akbar MT, Coffin S, de Belleroche J, Latchman DS (2003) Heat shock protein 27 delivered via a herpes simplex virus vector can protect neurones of the hippocampus against kainic acidinduced cell loss. Molecular Brain Research 111:91–103

    Article  CAS  PubMed  Google Scholar 

  9. Latchman DS (2001) Heat shock proteins and cardiac protection. Cardiovascular Research 51:637–646

    Article  CAS  PubMed  Google Scholar 

  10. Latchman DS (2003) Protective effect of heat shock proteins: potential for gene therapy. Gene Therapy and Molecular Biology 7:245–254

    Google Scholar 

  11. Lau S, Patnaik N, Sayen RS, Mestril R (1997) Simultaneous overexpression of two stress proteins in rat cardiomyocytes and myogenic cells confers protection against ischaemia-induced injury. Circulation 96:2287–2294

    CAS  PubMed  Google Scholar 

  12. Lee JE, Yenari MA, Sun GH, Xu L, Emond MR, Cheng D, Steinberg GK, Giffard RG (2001) Differential neuroprotection from human heat shock protein 70 overexpression in in vitro and in vivo models of ischaemia and ischaemia-like conditions. Experimental Neurology 170:129–139

    Article  CAS  PubMed  Google Scholar 

  13. Marber MS, Walker JM, Latchman DS, Yellon DM (1994) Myocardial protection following whole body heat stress in the rabbit is dependent on metabolic substrate and is related to the amount of the inducible 70kb Dalton heat shock protein. Journal of Clinical Investigation 93:1087–1094

    CAS  PubMed  Google Scholar 

  14. Mestril R, Giordano FJ, Conde AG, Dillmann WH (1996) Adenovirus-mediated gene transfer of a heat shockprotein 70 (hsp70i) protects against simulated ischaemia. JMCC 28:2351–2358

    CAS  Google Scholar 

  15. Murashov AK, Ul Haq I, Hill C, Park E, Smith M, Wang X, Wang X, Goldberg DJ, Wolgemuth DJ (2001) Crosstalk between p38, Hsp25 and Akt in spinal motor neurons after sciatic nerve injury. Brain Res Mol Brain Res 93(2):199–208

    CAS  PubMed  Google Scholar 

  16. Plumier JCL, Ross BM, Currie RW, Angelidis CE, Kazlaris H, Kollias G, Pagoulatos GN (1995) Transgenic mice expressing the human heat shock protein 70 have improved postischaemic myocardial recovery. Journal of Clinical Investigation 95:1854–1860

    CAS  PubMed  Google Scholar 

  17. Rane MJ, Pan Y, Singh S, Powel DW, Wu R, Cummins T, Chen Q, McLeish KR, Klein JB (2003) Heat shock protein 27 controls apoptosis by regulating Akt activation. Journal Biological Chemistry 278, 30:27828–27835

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. The Hatter Institute and Centre for Cardiology, Universitiy College London Hospitals and Medical School London, Grafton Way, London WC1E 6DB, UK

    Christopher A. Efthymiou, Mihaela M. Mocanu & Derek M. Yellon Prof.

  2. Division of Neuroscience and Psychological Medicine, Faculty of Medicine, Imperial College London, Charing Cross Hospital, London W6 8RF, UK

    Jackie de Belleroche & Dominic J. Wells

  3. Institute of Child Health, University College London, 30 Guilford Street, London WC1N 1EH, UK

    David S. Latchmann

Authors
  1. Christopher A. Efthymiou
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  2. Mihaela M. Mocanu
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  3. Jackie de Belleroche
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  4. Dominic J. Wells
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  5. David S. Latchmann
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  6. Derek M. Yellon Prof.
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Correspondence to Derek M. Yellon Prof..

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Efthymiou, C.A., Mocanu, M.M., de Belleroche, J. et al. Heat shock protein 27 protects the heart against myocardial infarction. Basic Res Cardiol 99, 392–394 (2004). https://doi.org/10.1007/s00395-004-0483-6

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  • DOI: https://doi.org/10.1007/s00395-004-0483-6

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