Abstract
Nuclear DNA contents of more than 100 important plant species were measured by flow cytometry of isolated nuclei stained with propidium iodide.Arabidopsis exhibits developmentally regulated multiploidy and has a 2C nuclear DNA content of 0.30 pg (145 Mbp/1C), twice the value usually cited. The 2C value for rice is only about three times that ofArabidopsis. Tomato has a 2C value of about 2.0 pg, larger than commonly cited. This survey identified several horticultural crops in a variety of families with genomes only two or three times as large asArabidopsis; these include several fruit trees (a pricot, cherry, mango, orange, papaya, and peach). The small genome sizes of rice and the horticultural plants should facilitate molecular studies of these crops.
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Abbreviations
- C:
-
DNA content of the unreplicated haploid chromosome complement
- CRBC:
-
chicken red blood cells
- CE:
-
chemical extraction
- FC:
-
flow cytometry
- FMD:
-
Feulgen microdensitometry
- RK:
-
reassociation kinetics
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An erratum to this article is available at http://dx.doi.org/10.1007/BF02672016.
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Arumuganathan, K., Earle, E.D. Nuclear DNA content of some important plant species. Plant Mol Biol Rep 9, 208–218 (1991). https://doi.org/10.1007/BF02672069
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DOI: https://doi.org/10.1007/BF02672069