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Organculture of adult mouse intestine

I. Morphological results after 24 and 48 hours of culture

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Explants of adult mouse intestine have been maintained in organ culture for 24 to 48 hr. The best results have been obtained with a mixture of DMEM-HEPES medium and NCTC-135 enriched with 10% fetal bovine serum. The morphology of the mucosa is well preserved at the light electron microscopic level: absorbing cells exhibit an increase in secondary lysosomes; goblet cells and Paneth cells remain active; numerous mitoses are observed in the crypts; and vigorous re-epithelization takes place on the margin of the explants.

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Authors and Affiliations

  1. Intestinal Biology Unit, Department of Anatomy and Cell Biology, Medical Center, University of Sherbrooke, J1H 5N4, Sherbrooke, PQ, Canada

    S. Ferland (Recipient of a studentship from the Medical Research Council of Canada) & J. S. Hugon

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This work was supported by a research grant from the Medical Research Council of Canada (to J. S. H.) and partly by the F.C.A.C. Québec (to J. S. H.).

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Ferland, S., Hugon, J.S. Organculture of adult mouse intestine. In Vitro 15, 278–287 (1979). https://doi.org/10.1007/BF02618952

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