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Cell size in aging monolayer cultures

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Summary

Changes in the size of the area covered by individual cultured WI-38 cells as the cultures age have been studied by using a new microphotographic paper cutout technique. This method is nondestructive and nonintrusive and avoids a number of artifacts which can occur in the measurement of suspended cells. The measurements reveal that the decreased cell yield of late passage cultures-reflects not only the appearance of a subpopulation of larger cells but also the failure of the cells to utilize all the growth surface available to them.

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Authors and Affiliations

  1. The Wistar Institute of Anatomy and Biology, 36th Street at Spruce, 19104, Philadelphia, Pennsylvania

    S. B. Greenberg, G. L. Grove & V. J. Cristofalo

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  1. S. B. Greenberg
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  2. G. L. Grove
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  3. V. J. Cristofalo
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Additional information

This work was supported in part by USPHS research grant AG-00378 and by a fellowship, AG-05019, from the National Institute on Aging.

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Greenberg, S.B., Grove, G.L. & Cristofalo, V.J. Cell size in aging monolayer cultures. In Vitro 13, 297–300 (1977). https://doi.org/10.1007/BF02616174

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