Summary
Escherichia coli strains carrying ribosomal and nonribosomal mutations were used to perform mapping experiments to determine the relative order of various genes which code for ribosomal proteins. The strains used carried the two nonribosomal mutations, crp and aro E, which seem to flank the ribosomal genes. They also carried mutations which conferred resistance to erythromycin, kanamycin, neomycin, spectinomycin, and streptomycin, and mutations which conferred dependence on or independence from streptomycin. A series of transduction experiments together with some data from the literature suggest the following order: crp, k12, str, str i (ram), eryA, eryB, spc, nek, aroE. Thus far no nonribosomal gene is located between ribosomal genes, and at least some 50S ribosomal genes are interspersed between 30S genes.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Apirion, D., Schlessinger, D.: Coresistance to neomycin and kanamycin by mutations in an Escherichia coli locus that affects ribosomes. J. Bact. 96, 768–776 (1968a)
Apirion, D., Schlessinger, D.: Mapping and complementation of three genes specifying 30S ribosomal components in Escherichia coli. J. Bact. 96, 1431–1432 (1968b)
Birge, E.A., Kurland, C.G.: Altered ribosomal protein in streptomycin depedent Escherichia coli. Science 166, 1282–1284 (1969)
Birge, E.A., Kurland, C.G.: Reversion of a streptomycin dependent strain of Escherichia coli. Molec. gen. Genet. 109, 356–369 (1970)
Bollen, A., Eaelen, M., Lecocq, J.P., Herzog, A., Zengel, J., Kahan, L., Normura, M.: The structural gene for the ribosomal protein S18 in Escherichia coli. Genetic studies on a mutant having an alteration in the protein S18. J. molec. Biol. 76, 463–472 (1973)
Bollen, A., Helser, T., Yamada, T., Davies, J.: Altered ribosomes in antibiotic resistant mutants of E. coli. Cold Spr. Harb. Symp. quant. Biol. 34, 95–100 (1969)
Dekio, S., Takata, R., Osawa, S.: Genetic studies of the ribosomal proteins in Escherichia coli. Molec. gen. Genet. 109, 131–141 (1970)
Epstein, W., Kim, B.: Potassium Transport loci in Escherichia coli K-12. J. Bact. 108, 639–644 (1971)
Gulløv, K., von Meyenburg, K., Molin, S.: The size of transcriptional units for ribosomal proteins in Escherichia coli. Rates of synthesis of ribosomal proteins during nutritional shift-up. Molec. gen. Genet. 130, 271–274 (1974)
Hasenbank, R., Guthrie, C., Stoffler, G., Wittmann, H.G., Rosen, L., Apirion, D.: Electrophoretic and immunological studies on ribosomal proteins of 100 Escherichia coli revertants from streptomycin dependence. Molec. gen. Genet. 127, 1–18 (1973)
Kaltschmidt, E., Stoffler, G., Dzionara, M., Wittmann, H.G.: Ribosomal proteins-comparative studies of ribosomal proteins of four strains of Escherichia coli. Molec. gen. Genet. 109, 303–308 (1970)
Lennette, E.T., Apirion, D.: Genetic analysis of an Escherichia coli syndrome. J. Bact. 108, 1322–1328 (1971)
Luzzatto, L., Schlessinger, D., Apirion, D.: Escherichia coli: high resistance or depedence on streptomycin produced by the same allele. Science 161, 478–479 (1968)
Mayuga, C., Meier, D., Wang, T.: Escherichia coli: The K12 ribosomal protein and the streptomycin region of the chromosome. Biochem. biophys. Res. Commun. 33, 203–206 (1968)
Molin, S., von Meyenburg, K., Gulløv, K., Maaløe, O.: The size of transcriptional units for ribosomal proteins in Escherichia coli. Molec. gen. Genet. 129, 11–26 (1974)
Nomura, M., Engback, F.: Expression of ribosomal protein genes as analyzed by bacteriophage Mu-induced mutations. Proc. nat. Acad. Sci. (Wash.) 69, 1526–1530 (1972)
Ozaki, M., Mizushima, S., Nomura, M.: Identification and functional characterization of the protein controlled by the streptomycin-resistant locus in E. coli. Nature (Lond.) 222, 333–339 (1969)
Rossett, R., Gorini, L.: A ribosomal ambiguity mutation. J. molec. Biol. 39, 95–112 (1969)
Saltzman, L., Brown, M., Apirion, D.: Functional interdependence among ribosomal elements as revealed by genetic analysis. Molec. gen. Genet. (in press)
Sparling, P., Blackman, E.: Mutation to erythromycin dependence in Escherichia coli K-12. J. Bact. 116, 74–83 (1973)
Wittmann, H., Stoffler, G., Apirion, D., Rosen, L., Tanaka, K., Tamaki, M., Takata, R., Dekio, S., Otaka, E., Osawa, S.: Biochemical and genetic studies on two different types of erythromycin resistant mutants of Escherichia coli with altered ribosomal proteins. Molec. gen. Genet. 127, 175–189 (1973)
Author information
Authors and Affiliations
Additional information
Communicated by E. Bautz
Rights and permissions
About this article
Cite this article
Brown, M.E., Apirion, D. Mapping a cluster of ribosomal genes in Escherichia coli . Molec. gen. Genet. 133, 317–327 (1974). https://doi.org/10.1007/BF00332707
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00332707