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Transmembrane currents in frog olfactory cilia

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Summary

We have measured transmembrane currents in intact single cilia from frog olfactory receptor neurons. A single cilium on a neuron was sucked into a patch pipette, and a high-resistance seal was formed near the base of the cilium. Action potentials could be induced by applying suction or a voltage ramp to the ciliary membrane. A transient current was seen in some cells on stimulation with odorants. After excision from the cell, most of the cilia showed increased conductance in a bath containing cAMP, indicating that the cytoplasmic face of the ciliary membrane was accessible to the bath. The estimated resistance of a single cilium was surprisingly low.

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Authors and Affiliations

  1. Department of Anatomy and Cell Biology, University of Cincinnati, 45267-0521, Cincinnati, Ohio

    Steven J. Kleene & Robert C. Gesteland

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  1. Steven J. Kleene
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  2. Robert C. Gesteland
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Kleene, S.J., Gesteland, R.C. Transmembrane currents in frog olfactory cilia. J. Membrain Biol. 120, 75–81 (1991). https://doi.org/10.1007/BF01868593

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  • DOI: https://doi.org/10.1007/BF01868593

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