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Presence of a 16S rRNA pseudogene in the bi-molecular plastid genome of the primitive brown alga Pylaiella littoralis. Evolutionary implications

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Summary

The plastid genome of the brown alga Pylaiella littoralis (L.) Kjellm. is composed of two different circular DNA molecules: the largest carries two rrn operons, and the smallest, only one copy of both 16S and 23S rDNAs. 16S rDNA copies located on both molecules have been cloned and their nucleotide sequences determined: they are 65% homologous to one another. The expression of these genes was assayed by hybridizing in vivo labelled P. littoralis rRNAs to both clones, and specific oligonucleotides to total RNA from P. littoralis. Results indicate that the 16S rDNA copy located on the small molecule is a pseudogene. Comparisons of the functional gene with other 16S rRNA genes shows that chloroplasts from green plants emerged earlier from the cyanobacterial lineage than Euglena gracilis and Pylaiella littoralis plastids.

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References

  • Baudin F, Ehresmann C, Romby P, Mougel M, Colin J, Lempereur L, Bachellerie JP, Ebel JP, Ehresmann B (1987) Biochimie 69:1081–1096

    Google Scholar 

  • Bonen L, Doolittle WF (1976) Nature 261:669–673

    Google Scholar 

  • Brennicke A, Möller S, Blanz PA (1985) Mol Gen Genet 198: 404–410

    Google Scholar 

  • Briat JF, Dron M, Loiseaux S, Mache R (1982) Nucleic Acids Res 10:6865–6878

    Google Scholar 

  • Brimacombe R (1984) TIBS 9:273–277

    Google Scholar 

  • Brimacombe R, Atmadja J, Stiege W, Schüer D (1988) J Mol Biol 199:115–136

    Google Scholar 

  • Dams E, Hendriks L, Van de Peer Y, Neefs JM, Smits G, Vandenbempt I, De Wachter R (1988) Nucleic Acids Res 16:r87-r173

    Google Scholar 

  • Dretzen G, Bellard M, Sassone-Corsi P, Chambon P (1981) Anal Biochem 112:295–298

    Google Scholar 

  • Elwood HJ, Olsen GJ, Sogin ML (1985) Mol Biol Evol 2:399–410

    Google Scholar 

  • Fox GE, Stackebrandt E, Hespell RB, Gibson J, Maniloff J, Dyer TA, Wolfe RS, Balch WE, Tanner RS, Magrum LJ, Zablen LB LB, Blakemore R, Gupta R, Bonen L, Lewis BJ, Stahl DA, Luehrsen KR, Chen KN, Woese CR (1980) Science 209:457–463

    Google Scholar 

  • Gibbs SP (1981) Ann NY Acad Sci 361:193–207

    Google Scholar 

  • Graf L, Roux E, Stutz E, Kössel H (1982) Nucleic Acids Res 10: 6369–6381

    Google Scholar 

  • Greenwood AD, Griffiths HB, Santore UJ (1977) Br Phycol J 12:119

    Google Scholar 

  • Hellman L, Pettersson U (1987) Gene Anal Techniques 4:9–13

    Google Scholar 

  • Herzog M, Maroteaux L (1986) Proc Natl Acad Sci USA 83: 8644–8648

    Google Scholar 

  • Hori H, Osawa S (1979) Proc Natl Acad Sci USA 76:381–385

    Google Scholar 

  • Horrander J, Kempe T, Messing J (1983) Gene 26:101–106

    Google Scholar 

  • Jacobs WF, Santer M, Dahlberg AE (1987) Proc Natl Acad Sci USA 84:4757–4761

    Google Scholar 

  • Koller B, Roux E, Montandon PE, Stutz E (1988) Plant Mol Biol 10: 339–347

    Google Scholar 

  • Kössel H, Natt E, Strittmatter G, Fritzsche E, Gozdzicka-Jozefiak A, Przybyl D (1985) In: Van Vloten-Doting L, Groot GSP, Hall TC (eds) Molecular form and function of the plant genome. Plenum Press, New York, pp 183–198

    Google Scholar 

  • Kuhsel M, Kowallik KV (1987) Mol Gen Genet 207:361–368

    Google Scholar 

  • Leinfelder W, Jarsch M, Böck A (1985) Syst Appl Microbiol 6: 164–170

    Google Scholar 

  • Li N, Cattolico RA (1987) Mol Gen Genet 209:343–351

    Google Scholar 

  • Loiseaux S, Rozier C (1987) Rev Algol 13: 333–340

    Google Scholar 

  • Loiseaux S, Mache R, Rozier C (1979) Physiol Vég 17:619–629

    Google Scholar 

  • Loiseaux-de Goër S, Markowicz Y, Dalmon J, Audren H (1988) Curr Genet 14:155–162

    Google Scholar 

  • Lonsdale DM (1984) Plant Mol Biol 3:201–206

    Google Scholar 

  • Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning, a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY

    Google Scholar 

  • Markowicz Y, Mache R, Loiseaux-de Goër S (1988) Plant Mol Biol 10:465–469

    Google Scholar 

  • McCarroll R, Olsen GJ, Stahl YD, Woese CR, Sogin ML (1983) Biochemistry 22:5858–5868

    Google Scholar 

  • Mougel M, Eyermann F, Westhof E, Romby P, Expert-Bezançon A, Ebel JP, Ehresman B, Ehresman C (1987) J Mol Biol 198:91–107

    Google Scholar 

  • Noller HF, Woese CR (1981) Science 212:403–411

    Google Scholar 

  • Noller HF (1984) Annu Rev Biochem 53:119–162

    Google Scholar 

  • Noller HF, Asire M, Barta A, Douthwaite S, Goldstein T, Gutell RR, Moazed D, Normanly J, Prince JB, Stern S, Triman K, Turner S, Van Stolk B, Wheaton V, Weiser B, Woese CR (1986) In: Hardesty B, Kramer G (eds) Structure, function, and genetics of ribosomes. Springer, Berlin Heidelberg New York, pp 143–163

    Google Scholar 

  • Ofengand J, Ciesiolka J, Denman R, Nurse K (1986) In: Hardesty B, Kramer G (eds) Structure, function, and genetics of ribosomes. Springer, Berlin Heidelberg New York, pp 473–494

    Google Scholar 

  • Palmer JD (1985) Annu Rev Genet 19:325–354

    Google Scholar 

  • Reith M, Cattolico RA (1986) Proc Natl Acad Sci USA 83:8599–8603

    Google Scholar 

  • Roux E, Graf L, Stutz E (1983) Nucleic Acids Res 11:1957–1968

    Google Scholar 

  • Schneider M, Darlix JL, Erickson J, Rochaix JD (1985) Nucleic Acids Res 13:8531–8541

    Google Scholar 

  • Schwarz Z, Kössel H (1979) Nature 279:520–522

    Google Scholar 

  • Schwarz Z, Kössel H (1980) Nature 283:739–742

    Google Scholar 

  • Sogin ML, Gunderson JH (1987) Ann NY Acad Sci 503:125–139

    Google Scholar 

  • Van Knippenberg PH (1986) In: Hardesty B, Kramer G (eds) Structure, function, and genetics of ribosomes. Springer, Berlin Heidelberg New York, pp 412–424

    Google Scholar 

  • Whitfeld PR, Herrmann RG, Bottomley W (1978) Nucleic Acids Res 5:1741–1751

    Google Scholar 

  • Yamada T, Shimaji M (1987) Mol Gen Genet 208:377–383

    Google Scholar 

Download references

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Authors and Affiliations

  1. Laboratoire de Biologie Moléculaire Végétale, CNRS UA 1178, Université J. Fourier, BP 53X, F-38041, Grenoble Cédex, France

    Yves Markowicz, Susan Loiseaux-de Goer & Regis Mache

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  1. Yves Markowicz
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  2. Susan Loiseaux-de Goer
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  3. Regis Mache
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Markowicz, Y., Loiseaux-de Goer, S. & Mache, R. Presence of a 16S rRNA pseudogene in the bi-molecular plastid genome of the primitive brown alga Pylaiella littoralis. Evolutionary implications. Curr Genet 14, 599–608 (1988). https://doi.org/10.1007/BF00434086

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