Summary
The order of 802 base pairs was established in a DNA segment containing the promoter for malPQ which is one of the three maltose operons, and the promoter for malT, the positive regulator gene of the maltose regulon. The determination of the amino-terminal sequence of the MalT protein allowed us to identify the beginning of the malT gene on the sequence. The position of the malP gene was deduced from the published amino-terminal sequence of maltodextrin phosphorylase. A total of 611 base pairs separate the initiation codons for these two genes, which are transcribed in opposite directions. This large intergenic region does not code for any polypeptide of significant size. The main features of this sequence are discussed in terms of the regulation known to operate on malT and malPQ expression.
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References
Backman K, Ptashne M, Gilbert W (1976) Construction of plasmids carrying the CI gene of bacteriophage λ. Proc Natl Acad Sci USA 73:4174–4178
Bedouelle H, Hofnung M (1982) A DNA sequence containing the control regions of the mal malEFG and malK-lamB operon in Escherichia coli K12. Mol Gen Genet 185:82–87
Berkner KL, Folk WR (1977) Polynucleotide kinase exchange reaction. J Biol Chem 252:3176–3184
Casadaban MJ (1976) Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu. J Mol Biol 104:541–555
Débarbouillé M, Schwartz M (1979) The use of gene fusions to study the expression of malT the positive regulator gene of the maltose regulon. J Mol Biol 132:521–534
Débarbouillé M, Shuman HA, Silhavy TJ, Schwartz M (1978) Dominant constitutive mutations in malT, the positive regulator gene of the maltose regulon in Escherichia coli. J Mol Biol 124:359–371
Hofnung M, Schwartz M (1971) Muttions allowing growth on maltose of Escherichia coli K12 strains with a deleted malT gene. Mol Gen Genet 122:117–132
Katz L, Kingsbury DK, Helinski DR (1973) Stimulation by cyclic adenosine monophosphate of plasmid deoxyribonucleic acid replication and catabolite repression of the plasmid deoxyribonucleic acid-protein complex. J Bacteriol 114:577–591
Maxam AM, Gilbert W (1980) Sequencing end-labeled DNA with base specific chemical cleavages. In: Grossman L, Moldave K (eds) Methods in enzymology, vol 65. Academic Press, New York, pp 499–560
Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory, New York, pp 431–435
Radloff R, Bauer W, Vinograd J (1967) A dye buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in Hela cells. Proc Natl Acad Sci USA 57:1514–1521
Raibaud O, Roa M, Braun Breton C, Schwartz M (1979) Structure of the malB region in Escherichia coli K12. I Genetic map of the malK-lamB operon. Mol Gen Genet 174:241–248
Raibaud O, Schwartz M (1980) Restriction map of the Escherichia coli malA region and identification of the malT product. J Bacteriol 143:761–771
Sanger F, Coulson AR (1978) The use of thin acrylamide gels for DNA sequencing. FEBS Lett 87:107–110
Schiltz E, Palm D, Klein MW (1980) N terminal sequences of Escherichia coli and potato phosphorylase. FEBS Lett 109:59–62
Schwartz D, Beckwith J (1970) Mutants missing a factor necessary for the expression of catabolite-sensitive operons in E. coli. In: Beckwith JR, Zipser D (eds) The lactose operon. Cold Spring Harbor Laboratory, New York, pp 417–422
Shine J, Dalgarno L (1975) Determinant of cistron specificity in bacterial ribosomes. Nature 254:34–38
Silhavy T, Brickman E, Bassford PJ Jr, Casadaban M, Shuman HA, Schwartz V, Guarente L, Schwartz M, Beckwith J (1979) Structure of the malB region in Escherichia coli K12 II Genetic map of the malEFG operon. Mol Gen Genet 174:249–259
Taniguchi T, O'Neill M, De Crombrugghe B (1979) Interaction site of Escherichia coli cyclic AMP receptor protein on DNA of galactose operon promoters. Proc Natl Acad Sci USA 76:5090–5094
Tiollais P, Rambach A, Buc H (1974) Large scale purificaton of a bacterial gene FEBS Lett 48:96–100
Uhlin BE, Molin S, Gustafsson P, Nordström K (1979) Plasmids with temperature-dependent copy number for amplification of cloned genes and their products. Gene 6:91–106
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Debarbouille, M., Cossart, P. & Raibaud, O. A DNA sequence containing the control sites for gene malT and for the malPQ operon. Molec Gen Genet 185, 88–92 (1982). https://doi.org/10.1007/BF00333795
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DOI: https://doi.org/10.1007/BF00333795