Summary
5S RNA from B. stearothermophilus and E. coli was reacted with NaIO4 and aniline to remove their 3′ terminal nucleoside. These modified 5S RNA molecules were then incorporated in B. stearothermophilus 50 S ribosomal subunits and tested for biological activities. 50 S ribosomes containing the modified 5S RNAs exhibited full activity and we therefore conclude, that the 3′ terminus of 5S RNA does not play an active role in protein synthesis.
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Communicated by P. Starlinger
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Erdmann, V.A., Doberer, H.G. & Sprinzl, M. Structure and function of 5S RNA: The role of the 3′ terminus in 5S RNA function. Molec. Gen. Genetics 114, 89–94 (1972). https://doi.org/10.1007/BF00332779
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DOI: https://doi.org/10.1007/BF00332779