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One-step transformation of yeast in stationary phase

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Summary

A fast yeast-transformation technique has been developed by adding thio compounds to alkali-ion based protocols and incubating at 45°C. This procedure is especially recommended for cells from stationary phase at a density up to 2.5×108 cells/ml. It involves only one step for the preparation and transformation of competent cells within 30 min. The yield was more than 104 transformants/μg plasmid DNA. This protocol is easy to scale up for many DNA samples and is also applicable for yeast cells from different types of storages.

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Authors and Affiliations

  1. Graduate Institute of Botany, National Taiwan University, Taipei, Republic of China

    Dz-Chi Chen & Tsong-Teh Kuo

  2. Institute of Botany, Academia Sinica, Taipei, Republic of China

    Tsong-Teh Kuo

  3. Institute of Molecular Biology, Academia Sinica, Taipei, Republic of China

    Dz-Chi Chen, Bei-Chang Yang & Tsong-Teh Kuo

Authors
  1. Dz-Chi Chen
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  2. Bei-Chang Yang
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  3. Tsong-Teh Kuo
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Communicated by A. Hinnen

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Chen, DC., Yang, BC. & Kuo, TT. One-step transformation of yeast in stationary phase. Curr Genet 21, 83–84 (1992). https://doi.org/10.1007/BF00318659

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  • DOI: https://doi.org/10.1007/BF00318659

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