Summary
Osteoclasts of medullary bone after several days of hypocalcaemic diet are substituted on the trabecular surface by active osteoblasts. The fate and the ultrastructure of the osteoclasts withdrawn from medullary bone surfaces in the course of a low calcium diet has been studied in serial semithin and ultrathin sections. The cytoplasmic surface of osteoclasts located in marrow compartments presents blebs and protrusions and the whole cell is often irregularly branched in several directions. A large amount of granular endoplasmic reticulum is accumulated at the cell periphery; often the cisternae are distended to form vesicles with an inner core of dense material. Osteoclasts seem to divide into mono or polynucleated smaller units.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bloom W, Bloom HA, McLean FC (1941) Calcification and ossification. Medullary bone changes in the reproductive cycle of female pigeon. Anat Rec 81:443–475
Bloom HA, Domm LV, Nalbandov AV, Bloom W (1958) Medullary bone of laying chickens. Am J Anat 102:411–453
de Bernard B, Stagni N, Camerotto R, Vittur F, Zanetti M, Zambonin Zallone A, Teti A (1980) Influence of calcium depletion on medullary bone of laying hens. Calcif Tissue Int 32:221–228
Gothlin G, Ericsson JLE (1973) On the histogenesis of the cells in fracture callus. Electron microscopic autoradiographic observations in parabiotic rats and studies on labelled monocytes. Virchows Arch BZ 12:318–329
Gothlin G, Ericsson JLE (1976) The osteoclast. Review of ultrastructure, origin and structure-function relationship. Clin Orthop 120:201–228
Hall BK (1975) The origin and fate of osteoclasts. Anat Rec 183:1–12
Hancox NM, Boothroyd B (1961) Motion picture and electron microscopic studies on the embryonic avian osteoclast. J Biophys Cytol 11:651–661
Holtrop ME, King GJ (1977) The ultrastructure of the osteoclast and its functional implications. Clin Orthop 123:177–196
Holtrop ME, Raisz LG, Simons HA (1974) The effect of parathyroid hormone, colchicine and calcitonin on the ultrastructure and activity of osteoclasts in organ culture. J Cell Biol 60:346–355
Jones SG, Boyde A (1977) Some morphological observations on osteoclasts. Cell Tissue Res 185:387–397
Jotereau FV, Le Douarin NM (1978) The developmental relationship between osteocyte and osteoclasts: A study using the Quail-Chick nuclear marker in endochondral ossification. Dev Biol 63:253–265
Kallio DM, Garant PR, Minkin C (1972) Ultrastructural effect of calcitonin on osteoclasts in tissue culture. J Ultrastruct Res 39:205–216
Kember NF (1960) Cell division in endochondral ossification. A study of cell proliferation in rat bones by the method of tritiated autoradiography. J Bone Joint Surg 42B:824–839
Lucht U (1972a) Osteoclasts and their relationship to bone as studied by electron microscopy. Z Zellforsch 135:211–228
Lucht U (1972b) Cytoplasmic vacuoles and bodies of the osteoclast: An Electron microscope study. Z Zellforsch 135:229–244
Lucht U (1973) Effect of calcitonin on osteoclasts in vivo. An ultrastructural and histochemical study. Z Zellforsch 145:75–87
Luk SC, Nopajaroonsri C, Simon GT (1974) The ultrastructure of the endosteum: A topographic study in young adult rabbits. J Ultrastruct Res 46:165–183
Loutit JF, Sansom JM (1976) Osteopetrosis of microphtalmic mice: A defect of the haematopoietic stem cell? Calc. Tissue Res 20:251–259
Marks Jr SC, Walker DG (1976) Mammalian osteopetrosis — A model for studying cellular and humoral factors in bone resorption. In: The biochemistry and physiology of bone. Vol IV. Calcification and Physiology (GH, ed) Chap 6. New York Academic Press
Miller SC (1977) Osteoclast cell-surface changes during the egg-laying cycle in Japanese quail. J Cell Biol 75:104–118
Miller SC (1978) Rapid activation of the medullary bone osteoclasts cell surface by parathyroid hormone. J Cell Biol 76:615–618
Mueller WJ, Schraer H, Schraer R (1974) Calcium metabolism and skeletal dynamics of laying pullets. J Nutr 84:20–26
Simmons DJ, Kahn AJ (1979) Cell lineage in fracture healing in Clinic Bone Graft. Calcif Tissue Int 27:247–253
Taylor TG, Bélanger LF (1969) The mechanism of bone resorption in laying hens. Calcif Tissue Res 4:162–173
Taylor TG, Moore JH (1954) Skeletal depletion in hens laying on a low calcium diet. Brit J Nutr 8:112–124
Taylor TG, Morris TR, hertlendy F (1962) The effect of putuitary hormones on ovulation in calcium deficient pullets. Vet Rec 74:123–125
Teitelbaum SL, Steward CC, Kahn AJ (1979) Rodent peritoneal macrophages as bone resorbing cells. Calcif Tissue Int 27:255–261
Teti A, Pace G, Zambonin Zallone A (1981) Isolamento di osteoclasti da osso follicolinico di Uccelli mediante sedimentazione a gravità unitaria. Boll Soc Ital Biol Sper 53:8–14
Urist MR (1959) The effect of calcium deprivation upon the blood adrenal cortex, ovary and skeleton in the domestic fowl. Rec Prog Hormone Res 15:455–477
Walker DG (1975) Control of bone resorption by haematopietic tissue. The induction and reversal of congenital osteopetrosis in mice through use of bone marrow and splenic transplant. J Exp Med 142:651–663
Watari N (1974) Intracisternal inclusion bodies induced in animals. Their transformation and significance. J. Electron Microsc 23:255–268
Young RW (1962) Cell proliferation and specialization during endochondral osteogenesis in young rats. J Cell Biol 14:357–370
Zambonin Zallone A, Mueller WJ (1969) Medullary bone of laying hens during calcium depletion and repletion. Calcif Tissue Res 4:136–146
Zambonin Zallone A, Teti A (1977) Modificazioni della struttura delle ossa in galline ovaiole a dieta ipocalcica. Boll Soc Ital Biol Sper 53:1073–1076
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Zallone, A.Z., Teti, A. The osteoclasts of hen medullary bone under hypocalcaemic conditions. Anat Embryol 162, 379–392 (1981). https://doi.org/10.1007/BF00301864
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00301864