Summary
Improved methods for rearing and screening large numbers of flies permitted the recovery of 10 mutations exhibiting a reversible temperature-dependent adult paralysis among 1.1×106 flies tested. Of the 10 mutations, two were allelic to para ts,two were alleles in a new locus, stoned (stn), and six fell into a third area, the shibire (shi) locus. Several of the shi alleles cause embryonic, larval and adult paralysis at 29° C as well as structural anomalies of various tissues. In addition to the ts mutations, several non-conditional mutations affecting adult movement were recovered.
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Communicated by Ch. Auerbach
This work was supported by the National Research Council of Canada, grant A-1764, National Cancer Institute of Canada, grant 6051.
Medical Research Council of Canada Postdoctoral Fellow.
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Grigliatti, T.A., Hall, L., Rosenbluth, R. et al. Temperature-sensitive mutations in Drosophila melanogaster . Molec. Gen. Genet. 120, 107–114 (1973). https://doi.org/10.1007/BF00267238
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DOI: https://doi.org/10.1007/BF00267238