Summary
Streptomyces griseus TÜ 6 produces the sideromycin antibiotic albomycin δ2 in concentrations of approximately 1 mg/l. The production depends on the phosphate, iron, and ornithine concentrations in the medium. In optimized conditions, the production of albomycin could be increased to 25 mg/l in a fedbatch fermentation. Isolation and purification could be achieved by reversed-phase and size-exclusion chromatography and preparative high-performance liquid chromatography (HPLC). The detection limit in quantitative determination of albomycin by HPLC was reached at a concentration of 1 μg/ml, which was 100 times less sensitive than biological testing, but this method, although time-consuming, was more selective.
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Fiedler, H.P., Walz, F., Döhle, A. et al. Albomycin: Studies on fermentation, isolation and quantitative determination. Appl Microbiol Biotechnol 21, 341–347 (1985). https://doi.org/10.1007/BF00249977
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DOI: https://doi.org/10.1007/BF00249977