Abstract
Opium poppy latex contains a group of laticifer-specific, low-molecular-weight polypeptides called major latex proteins (MLPs). Here we describe a new member of the MLP gene family (gMLP 22) which shares 79.6% nucleotide and 84.6% amino acid sequence identity with previously isolated clones. DNA gel blot analysis indicates that the MLPs are encoded by at least eight genes which are divided into two subfamilies. The expression pattern for each subfamily, as seen in RNA gel blots, appears to be identical and laticifer-specific.
This is a preview of subscription content, log in via an institution to check access.
References
Griffing LP, Nessler CL: Immunolocalization of the major latex proteins in developing laticifers of opium poppy (Papaver somniferum L.). J Plant Physiol 134: 357–363 (1989).
Nessler CL: Comparative analysis of the major latex proteins of opium poppy. J Plant Physiol 132: 588–592 (1988).
Nessler CL, Allen RD, Galewsky S: Identification and characterization of latex specific proteins in opium poppy. Plant Physiol 74: 499–504 (1985).
Nessler CL, Kurz WGW, Pelcher LE: Isolation and analysis of the major latex protein genes of opium poppy. Plant Mol Biol 15: 951–953 (1990).
Nessler CL, Mahlberg PG: Laticifers in organs redifferentiated from Papaver somniferum callus. Can J Bot 57: 675–685 (1979).
Nessler CL, Vonder Haar RA: Cloning and expression analysis of DNA sequences for the major latex protein of opium poppy. Planta 180: 487–491 (1990).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nessler, C.L., Burnett, R.J. Organization of the major latex protein gene family in opium poppy. Plant Mol Biol 20, 749–752 (1992). https://doi.org/10.1007/BF00046460
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00046460