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Structural Biology of TRP Channels

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Mammalian Transient Receptor Potential (TRP) Cation Channels

Part of the book series: Handbook of Experimental Pharmacology ((HEP,volume 223))

Abstract

Membrane proteins remain challenging targets for structural biologists, despite recent technical developments regarding sample preparation and structure determination. We review recent progress towards a structural understanding of TRP channels and the techniques used to that end. We discuss available low-resolution structures from electron microscopy (EM), X-ray crystallography, and nuclear magnetic resonance (NMR) and review the resulting insights into TRP channel function for various subfamily members. The recent high-resolution structure of TRPV1 is discussed in more detail in Chapter 11. We also consider the opportunities and challenges of using the accumulating structural information on TRPs and homologous proteins for deducing full-length structures of different TRP channel subfamilies, such as building homology models. Finally, we close by summarizing the outlook of the “holy grail” of understanding in atomic detail the diverse functions of TRP channels.

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Acknowledgments

We thank members of the Gaudet lab, especially Christina Zimanyi, Sriram Srikant, and Jeffrey MacArthur for feedback and discussions on the manuscript. This work was supported by the National Institutes of Health (Grant R01 GM081340 to R.G.), and U.A.H. is the recipient of an EMBO Long-Term Fellowship.

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  1. Harvard University, Cambridge, MA, USA

    Ute A. Hellmich & Rachelle Gaudet

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  1. Ute A. Hellmich
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Correspondence to Ute A. Hellmich or Rachelle Gaudet .

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  1. Laboratory Ion Channel Research, Campus Gasthuisberg, KU Leuven Department, Cell Mol Medicin, Leuven, Belgium

    Bernd Nilius

  2. Pharmakologie und Toxikologie, Universität des Saarlandes, Institut für Experimentelle u. Klinische, Homburg, Germany

    Veit Flockerzi

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Hellmich, U.A., Gaudet, R. (2014). Structural Biology of TRP Channels. In: Nilius, B., Flockerzi, V. (eds) Mammalian Transient Receptor Potential (TRP) Cation Channels. Handbook of Experimental Pharmacology, vol 223. Springer, Cham. https://doi.org/10.1007/978-3-319-05161-1_10

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