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KIR Genotyping and Analysis

Genotyping and Disease Association Analysis

  • Protocol
Innate Immunity

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 415))

Summary

The genes encoding the killer immunoglobulin-like receptors (KIR) are situated within a segment of DNA that has undergone expansion and contraction over time due in large part to unequal crossing over. Consequently, individuals exhibit considerable haplotypic variation in terms of gene content. The highly polymorphic human leukocyte antigen (HLA) class I loci encode ligands for the KIR; thus, it is not surprising that KIR genes also show significant allelic polymorphism. As a result of the receptor–ligand relationship between KIR and HLA, functionally relevant KIR–HLA combinations need to be considered in the analysis of these genes as they relate to disease outcomes. This chapter will describe a genotyping method for identifying the presence/absence of the KIR genes and general approaches to data analysis in disease association studies.

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Author information

Authors and Affiliations

  1. Laboratory of Genomic Diversity, SAIC-Frederick, Inc., NCI-Frederick, Frederick, MD

    Maureen P. Martin & Mary Carrington

Authors
  1. Maureen P. Martin
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  2. Mary Carrington
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Editor information

Editors and Affiliations

  1. Centre d’Immunologie de Marseille-Luminy, INSERM, CNRS Université de la Méditerranée, Marseille, France

    Jonathan Ewbank  & Eric Vivier  & 

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© 2008 Humana Press Inc.

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Martin, M.P., Carrington, M. (2008). KIR Genotyping and Analysis. In: Ewbank, J., Vivier, E. (eds) Innate Immunity. Methods in Molecular Biology™, vol 415. Humana Press. https://doi.org/10.1007/978-1-59745-570-1_3

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  • DOI: https://doi.org/10.1007/978-1-59745-570-1_3

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-746-4

  • Online ISBN: 978-1-59745-570-1

  • eBook Packages: Springer Protocols

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