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Mouse Models of Accelerated Cellular Senescence

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Cellular Senescence

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1896))

Abstract

Senescent cells accumulate in multiple tissues as virtually all vertebrate organisms age. Senescence is a highly conserved response to many forms of cellular stress intended to block the propagation of damaged cells. Senescent cells have been demonstrated to play a causal role in aging via their senescence-associated secretory phenotype and by impeding tissue regeneration. Depletion of senescent cells either through genetic or pharmacologic methods has been demonstrated to extend murine lifespan and delay the onset of age-related diseases. Measuring the burden and location of senescent cells in vivo remains challenging, as there is no marker unique to senescent cells. Here, we describe multiple methods to detect the presence and extent of cellular senescence in preclinical models, with a special emphasis on murine models of accelerated aging that exhibit a more rapid onset of cellular senescence.

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Acknowledgments

This work was supported by the NIH grants P01-AG043376 (PDR, LJN), U19-AG056278 (PDR, LJN), and Glenn Foundation (LJN, CEB).

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Authors and Affiliations

  1. Institute on the Biology of Aging and Metabolism, University of Minnesota, Minneapolis, MN, USA

    Matthew J. Yousefzadeh Ph.D., Luise Angelini B.S., Paul D. Robbins Ph.D. & Laura J. Niedernhofer M.D., Ph.D.

  2. Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN, USA

    Matthew J. Yousefzadeh Ph.D., Luise Angelini B.S. & Paul D. Robbins Ph.D.

  3. Department of Molecular Medicine, The Scripps Research Institute, Jupiter, FL, USA

    Matthew J. Yousefzadeh Ph.D., Kendra I. Melos B.S., Luise Angelini B.S. & Paul D. Robbins Ph.D.

  4. Department of Molecular Genetics, The Ohio State University, Columbus, OH, USA

    Christin E. Burd Ph.D.

  5. Department of Cancer Biology and Genetics, The Ohio State University, Columbus, OH, USA

    Christin E. Burd Ph.D.

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  1. Matthew J. Yousefzadeh Ph.D.
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  2. Kendra I. Melos B.S.
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  3. Luise Angelini B.S.
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  4. Christin E. Burd Ph.D.
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  5. Paul D. Robbins Ph.D.
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  6. Laura J. Niedernhofer M.D., Ph.D.
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Correspondence to Laura J. Niedernhofer M.D., Ph.D. .

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  1. ERIBA, University Medical Center Groningen, Groningen, Groningen, The Netherlands

    Marco Demaria

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Yousefzadeh, M.J., Melos, K.I., Angelini, L., Burd, C.E., Robbins, P.D., Niedernhofer, L.J. (2019). Mouse Models of Accelerated Cellular Senescence. In: Demaria, M. (eds) Cellular Senescence. Methods in Molecular Biology, vol 1896. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8931-7_17

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