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Replication of Human Papillomavirus in Culture

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Cervical Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1249))

Abstract

Human papillomaviruses (HPV) are the major factor in causing cervical cancer as well as being implicated in causing oral and anal cancers. The life cycle of HPV is tied to the epithelial differentiation system, as only native virus can be produced in stratified human skin. Initially, HPV research was only possible utilizing recombinant systems in monolayer culture. With new cell culture technology, systems using differentiated skin have allowed HPV to be studied in its native environment. Here, we describe current research studying native virions in differentiated skin including viral assembly, maturation, capsid protein interactions, and L2 cross-neutralizing epitopes. In doing so, we hope to show how differentiating skin systems have increased our knowledge of HPV biology and identify gaps in our knowledge about this important virus.

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Acknowledgements

We thank members of the Meyers’ laboratory for many helpful discussions. This work was supported by grant RO1 AI 057988 (C.M.).

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Authors and Affiliations

  1. Department of Microbiology and Immunology H107, The Pennsylvania State University College of Medicine, Hershey, PA, 17033, USA

    Eric J. Ryndock, Jennifer Biryukov & Craig Meyers

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  1. Eric J. Ryndock
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  2. Jennifer Biryukov
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  3. Craig Meyers
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Correspondence to Craig Meyers .

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  1. Touro University California, Vallejo, California, USA

    Daniel Keppler

  2. Touro University California, Vallejo, California, USA

    Athena W. Lin

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Ryndock, E.J., Biryukov, J., Meyers, C. (2015). Replication of Human Papillomavirus in Culture. In: Keppler, D., Lin, A. (eds) Cervical Cancer. Methods in Molecular Biology, vol 1249. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2013-6_3

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  • DOI: https://doi.org/10.1007/978-1-4939-2013-6_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2012-9

  • Online ISBN: 978-1-4939-2013-6

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