Abstract
The genome of the murine coronavirus JHM is a single-stranded, non-seamented RNA with a molecular weight of 5.4 — 6.7 × 10. The genomic RNA is infectious and at least one third is polyadenylated (Lai and Stohlman, 1978; Wege et al., 1978). The virion contains six proteins, four of which are glycosylated (Wege et al., 1979; Siddell et al., 1981b), including high and low molecular weight glycoproteins and a phosphorylated nucleocapsid protein of 60,000 molecular weight (Stohlman and Lai, 1979; Siddell et al., 1981a). Associated with purified virions is a protein kinase activity which specifically phosphorylates pp60 in vitro (Siddell et al., 1981a).
Chapter PDF
Similar content being viewed by others
Keywords
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
References
Anderson, R., Cheley, S. and Haworth-Hatherell, H., (1979). Comparison of polypeptides of two strains of murine hepatitis virus. Virology 97, 492–494.
Bond, C.W., Leibowitz, J.L., and Robb, J.A., (1979). Pathogenetic murine coronaviruses. II. Characterization of virus specific proteins of murine coronaviruses JHMV and A59V. Virology 94, 371–384.
Cavanagh, D. (1981). Structural polypeptides of coronavirus IBV. Journal of General Virology, in the press.
Garwes, D.J. and Pocock, D.H. (1975). The polypeptide structure of transmissible gastroenteritis virus. Journal of General Virology 29, 25–34.
Laemmli, U.K., (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227: 680–685.
Lai, M.M.C. and Stohlman, S.A. (1978). RNA of mouse hepatitis virus. Journal of Virology 26: 236–248.
Macnaughton, M.R. (1980). The polypeptides of human and mouse coronaviruses. Archives of Virology 63, 75–80.
Pocock, D.J. and Garwes, D.H. (1977). The polypeptides of haemagglutinating encephalomyelitis virus and isolated subviral particles. Journal of Virology 37, 487–499.
Siddell, S.G., Wege H., Barthel, A. and ter Meulen, V. (1980). Coronavirus JHM: Cell-free Synthesis of Structural protein p 60. Journal of Virology 33, 10–17.
Siddell, S.G., Barthel, A. and ter Meulen, V. (1981a). Coronavirus JHM. A virion-associated protein kinase Journal of General Virology, in the press.
Siddell, S.G., Wege, H., Barthel, A. and ter Meulen, V. (1981b). Coronavirus JHM. Intracellular protein synthesis. Journal of General Virology, in the press.
Smith, A.E., Wheeler, T., Glanville, N. and Kääriäinen, L. (1974). Translation of Semliki Forest virus 42S RNA in a mouse cell-free system to give virus coat proteins. European Journal of Biochemistry 49, 101–110.
Spaan, W.J.M., Rottier, P.J.M., Horzinek, M.C. and van der Zeijst, B.A.M. (1981). Isolation and identification of virus specific mRNA’s in cells infected with mouse hepatitis virus (MHV-A59). Virology, in the press.
Stern, D.F. and Kennedy, S.I.T. (1980a). The coronavirus multiplication strategy. I. Identification and characterization of virus-specified RNA. Journal of Virology 34, 665–674.
Stern, D.F. and Kennedy, S.I.T. (1980b). Coronavirus Multiplication Strategy. II. Mapping the avian infectious bronchitis virus intracellular RNA species to the genome. Journal of Virology 36, 440–449.
Stohlman, S.A. and Lai, M.M.C. (1979). Phosphoproteins of murine hepatitis viruses. Journal of Virology 32, 672–675.
Sturman, L.S. (1977). Characterization of a coronavirus. I. Structural proteins: effect of preparative conditions on the migration of protein in polyacrylamide gels. Virology 77, 637–649.
Sturman, L.S. and Holmes, K.V. (1977). Characterization of a coronavirus. II. Glycoproteins of the viral envelope: tryptic peptide analysis. Virology 77, 650–660.
Wege, H., Müller, A. and ter Meulen, V. (1978). Genomic RNA of the murine coronavirus JHM. Journal of General Virology 41, 217–227.
Wege, H., Wege, H., Nagashima, K., and ter Meulen, V. (1979). Structural polypeptides of the murine coronavirus JHM. Journal of General Virology 42, 37–47.
Wege, H., Siddell, S.G., Sturm, M. and ter Meulen, V. (1981). Coronavirus JHM. Characterization of intracellular viral RNA, Journal of General Virology, in the press.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1981 Springer Science+Business Media New York
About this chapter
Cite this chapter
Siddell, S., Wege, H., Barthel, A., ter Meulen, V. (1981). Intracellular Protein Synthesis and the in Vitro Translation of Coronavirus JHM mRNA. In: ter Meulen, V., Siddell, S., Wege, H. (eds) Biochemistry and Biology of Coronaviruses. Advances in Experimental Medicine and Biology, vol 142. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-0456-3_17
Download citation
DOI: https://doi.org/10.1007/978-1-4757-0456-3_17
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4757-0458-7
Online ISBN: 978-1-4757-0456-3
eBook Packages: Springer Book Archive