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The Pseudomonas aeruginosa Resistome: Permanent and Transient Antibiotic Resistance, an Overview

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Pseudomonas aeruginosa

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2721))

Abstract

One of the most concerning characteristics of Pseudomonas aeruginosa is its low susceptibility to several antibiotics of common use in clinics, as well as its facility to acquire increased resistance levels. Consequently, the study of the antibiotic resistance mechanisms of this bacterium is of relevance for human health. For such a study, different types of resistance should be distinguished. The intrinsic resistome is composed of a set of genes, present in the core genome of P. aeruginosa, which contributes to its characteristic, species-specific, phenotype of susceptibility to antibiotics. Acquired resistance refers to those genetic events, such as the acquisition of mutations or antibiotic resistance genes that reduce antibiotic susceptibility. Finally, antibiotic resistance can be transiently acquired in the presence of specific compounds or under some growing conditions. The current article provides information on methods currently used to analyze intrinsic, mutation-driven, and transient antibiotic resistance in P. aeruginosa.

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Acknowledgments

Work in the laboratory is supported by MCIN/AEI /10.13039/501100011033 – grant PID2020-113521RB-I00 LEOS is supported by a postdoctoral fellowship from Consejo Nacional de Ciencia y Tecnología (CONACyT-México). The funders had no role in study design, or the decision to submit the work for publication.

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All authors contributed to the design of the structure and the writing of the review. All authors have read and approved the submitted version of the article.

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  1. Centro Nacional de Biotecnología, CSIC, Madrid, Spain

    Fernando Sanz-García, Pablo Laborda, Luz Edith Ochoa-Sánchez, José Luis Martínez & Sara Hernando-Amado

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  1. Fernando Sanz-García
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  2. Pablo Laborda
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Correspondence to José Luis Martínez .

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  1. Department of Biosciences, Università degli Studi di Milano, Milan, Milano, Italy

    Giovanni Bertoni

  2. Department of Biosciences, Università degli Studi di Milano, Milan, Milano, Italy

    Silvia Ferrara

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Sanz-García, F., Laborda, P., Ochoa-Sánchez, L.E., Martínez, J.L., Hernando-Amado, S. (2024). The Pseudomonas aeruginosa Resistome: Permanent and Transient Antibiotic Resistance, an Overview. In: Bertoni, G., Ferrara, S. (eds) Pseudomonas aeruginosa. Methods in Molecular Biology, vol 2721. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3473-8_7

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