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Transthyretin

Antibody: CPTC-TTR-1
Background

Catalog Number:

CPTC-TTR-1

RRID:

AB_2617360

Target Antigen:

Transthyretin

Isotype:

IgG2a

Species:

Mouse Monoclonal Antibody

Last Updated:

09/09/2021

Antigen Recognition(s):

Recombinant Full-length, Endogenous

SOP:

Purchase
External Links
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (163.9 KB)

CPTC-TTR-1 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC NCI60

CPTC-TTR-1 IHC NCI60

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Micro-Array(TMA) core of breast cancer showing cytoplasmic staining using Antibody CPTC-TTR-1. Titer: 1:8000 Click image to enlarge

CPTC-TTR-1 IHC Tissue

Result: Positive

Tissue Micro-Array(TMA) core of breast cancer showing cytoplasmic staining using Antibody CPTC-TTR-1. Titer: 1:8000


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-TTR-1 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-TTR-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - HPA - tissue or cell lysate
Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). Band of predicted size in kDa (+/-20%) with additional bands present. Analysis performed using a standard panel of samples. Antibody dilution: 1:500. Click image to enlarge

CPTC-TTR-1 Evaluation by the Human Protein Atlas

Result: Presumed Positive (with additional bands)

Results provided by the Human Protein Atlas (www.proteinatlas.org). Band of predicted size in kDa (+/-20%) with additional bands present. Analysis performed using a standard panel of samples. Antibody dilution: 1:500.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-TTR-1 as primary Ab against TTR (Ag 11118) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-TTR-1 Western blot

Result: Positive

Western Blot using CPTC-TTR-1 as primary Ab against TTR (Ag 11118) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antigen: Transthyretin
Background

NCI Identification Number:

11118

Antigen Name:

Transthyretin

CPTC Name:

CPTC-TTR

Aliases:

Transthyretin; PALB; HsT2651; prealbumin, amyloidosis type 1; ATTR; TBPA; CTS; CTS1; carpal tunnel syndrome 1; thyroxine-binding prealbumin; prealbumin

Function:

Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.

Chromosomal Localization:

18q12.1

Expression System:

E. Coli

Accession Number:

BC005310

UniProt Accession Number:

P02766

DNA Source:

HsCD00004170

Immunogen:

Recombinant Full Length Protein

Vector Name:

pMCSG7

Extinction Coefficient:

18450

Buffers:

PBS without Ca++ and Mg++

Expressed Sequence:

SNAPTGTGESKCPLMVKVLDAVRGSPAINVAVHVFRKAADDTWEPFASGK
TSESGELHGLTTE

Native Sequence:

MASHRLLLLCLAGLVFVSEAGPTGTGESKCPLMVKVLDAVRGSPAINVAV
HVFRKAADDTWEPFASGKTSESGELHGLTTEEEFVEGIYKVEIDTKSYWK
ALGISPFHEHAEVVFTANDSGPRRYTIAALLSPYSYSTTAVVTNPKE

Calculated Isoelectric Point:

5.35

Molecular Weight:

13704

Last Updated:

08/03/2012

Links

Characterization Data

Gel

Click to enlarge image PAGE of TTR (rAg 11118) with molecular weight standards in lane 1
Click image to enlarge

CPTC-TTR PAGE

PAGE of TTR (rAg 11118) with molecular weight standards in lane 1

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SOPs

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