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Signal Transducer And Activator Of Transcription 1 Peptide 3

Antibody: CPTC-STAT1-3
Background

Catalog Number:

CPTC-STAT1-3

Target Antigen:

Signal Transducer And Activator Of Transcription 1 Peptide 3

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

09/05/2024

Antigen Recognition(s):

Peptide, Phosphorylation

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Characterization Data [Compare Characterization Data]
  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-STAT1-3. The antibody specifically recognizes the phosphorylated peptide GTG(pY)IK. Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from synthetic peptide. Click image to enlarge

CPTC-STAT1-3 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-STAT1-3. The antibody specifically recognizes the phosphorylated peptide GTG(pY)IK.
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from synthetic peptide.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-STAT1-3 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide

CPTC-STAT1-3 Western Blot (Recombinant Protein)

Result: Negative

Western blot using CPTC-STAT1-3 as primary antibody against human signal transducer and activator of transcription 1 recombinant protein. The expected molecular weight is 87.2 kDa.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-STAT1-3 Simple Western (cell lysates)

Result: Negative

Automated WB (Simple Western) using CPTC-STAT1-3 as primary antibody against the whole lysate of PBMC, HeLa, Jurkat, A549, MCF7 and H226. Expected MW is 97.3 KDa. The antibody does not recognize the target in Jurkat, A549 and H226 lysates. The same cell lysates were probed with an anti-CytC antibody.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

Title: CPTC-STAT1-3 Western Blot (Cell Lysate)

Result: Negative

Western blot using CPTC-STAT1-3 as primary antibody against human HeLa (2), Jurkat (3), A549 (4), MCF7 (5) and H226 (6) whole cell lysates. The expected molecular weight is 87.3 kDa.


Characterization SOP Files

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Antigen: Signal Transducer And Activator Of Transcription 1 Peptide 3
Background

NCI Identification Number:

00396

Antigen Name:

Signal Transducer And Activator Of Transcription 1 Peptide 3

CPTC Name:

CPTC-STAT1 Peptide 3

Aliases:

Signal Transducer And Activator Of Transcription 1; Transcription Factor ISGF-3 Components P91/P84; Signal Transducer And Activator Of Transcription 1-Alpha/Beta; Signal Transducer And Activator Of Transcription 1, 91kDa; Signal Transducer And Activator Of Transcription 1, 91kD; ISGF-3; STAT91; CANDF7; IMD31A; IMD31B; IMD31C; STAT1

Function:

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described.STAT1 (Signal Transducer And Activator Of Transcription 1) is a Protein Coding gene. Diseases associated with STAT1 include Immunodeficiency 31A and Immunodeficiency 31C. Among its related pathways are Common Cytokine Receptor Gamma-Chain Family Signaling Pathways and Interleukin-11 Signaling Pathway. Gene Ontology (GO) annotations related to this gene include DNA-binding transcription factor activity and protein homodimerization activity. An important paralog of this gene is STAT4.Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus (PubMed:28753426). ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated (PubMed:26479788). It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4.

Chromosomal Localization:

2q32.2

Accession Number:

NP_001371809.1

UniProt Accession Number:

P42224

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

GTG(pY)IK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

660

Last Updated:

09/02/2020

Links

Characterization Data

SOPs

No SOPs available.

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