Background
Catalog Number:
CPTC-MGMT-1
RRID:
AB_2890089
Target Antigen:
O-6-Methylguanine-DNA Methyltransferase Peptide 1
Isotype:
IgG
Species:
Rabbit Monoclonal Antibody
Last Updated:
02/09/2024
Antigen Recognition(s):
Peptide
Purchase
- DSHB CPTC-MGMT-1
Characterization Data [Compare Characterization Data]
IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (473.2 KB)
CPTC-MGMT-1 evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (473.2 KB)
CPTC-MGMT-1 evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
NCI 60 Protein Array
CTPC-MGMT-1 NCI60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-MGMT-1 Western Blot Recombinant Protein
Result: Negative
Western blot using CPTC-MGMT-1 as primary antibody against human O-6-methylguanine-DNA methyltransferase (MGMT) recombinant protein (lane 2). Expected molecular weight - 24.9 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Background
NCI Identification Number:
00378
Antigen Name:
O-6-Methylguanine-DNA Methyltransferase Peptide 1
CPTC Name:
CPTC-MGMT Peptide 1
Aliases:
O-6-Methylguanine-DNA Methyltransferase; Methylated-DNA--Protein-Cysteine Methyltransferase; 6-O-Methylguanine-DNA Methyltransferase; O-6-Methylguanine-DNA-Alkyltransferase; EC 2.1.1.63; O6-Methylguanine-DNA Methyltransferase; Methylguanine-DNA Methyltransferase; MGMT
Function:
Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. The protein encoded by this gene is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O(6)-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the genes promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma. MGMT (O-6-Methylguanine-DNA Methyltransferase) is a Protein Coding gene. Diseases associated with MGMT include Oligodendroglioma and Gliosarcoma. Among its related pathways are DNA Damage Reversal and Chromatin Regulation / Acetylation. Gene Ontology (GO) annotations related to this gene include calcium ion binding and damaged DNA binding.Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Protein arginine methyltransferases are enyzmes that catalyze the transfer of methyl groups from S-adenosylmethionine (SAM) to the arginine residues on histones and other proteins. The dysregulation of this methylation is critical in the development of certain cancers.
Chromosomal Localization:
10q26.3
Accession Number:
NP_002403.3
UniProt Accession Number:
P16455
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
GNPVPILIPCHR
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
1320
Last Updated:
09/02/2020
Links
- EnsEMBL - EnsEMBL MGMT
- Entrez - Entrez MGMT
- Uniprot - Uniprot P16455
Characterization Data
SOPs
No SOPs available.
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