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Addgene

pAKgfp1
(Plasmid #14076)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14076 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBBR1MCS4
  • Backbone size w/o insert (bp) 4942
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Green fluorescent protein
  • Alt name
    GFP
  • Alt name
    GFPmut3a
  • Species
    Aequorea victoria
  • Insert Size (bp)
    789

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAKgfp1 was a gift from Attila Karsi (Addgene plasmid # 14076 ; http://n2t.net/addgene:14076 ; RRID:Addgene_14076)

  • For your References section:

    Broad host range fluorescence and bioluminescence expression vectors for Gram-negative bacteria. Karsi A, Lawrence ML. Plasmid. 2007 May;57(3):286-95. doi: 10.1016/j.plasmid.2006.11.002 10.1016/j.plasmid.2006.11.002 PubMed 17207855
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