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Addgene

pAAV-CaMKII-Archon1-KGC-EGFP-ER2
(Plasmid #108419)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108419 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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This service is available to academics and nonprofits only.

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Backbone

  • Vector backbone
    pAAV-CaMKII
  • Total vector size (bp) 1602
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Archon1-KGC-EGFP-ER2
  • Species
    Synthetic
  • Insert Size (bp)
    1581
  • GenBank ID
    MG250280.1
  • Promoter CaMKII

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CaMKII-Fw
  • 3′ sequencing primer WPRE-Rv
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CaMKII-Archon1-KGC-EGFP-ER2 was a gift from Edward Boyden (Addgene plasmid # 108419 ; http://n2t.net/addgene:108419 ; RRID:Addgene_108419)

  • For your References section:

    A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters. Piatkevich KD, Jung EE, Straub C, Linghu C, Park D, Suk HJ, Hochbaum DR, Goodwin D, Pnevmatikakis E, Pak N, Kawashima T, Yang CT, Rhoades JL, Shemesh O, Asano S, Yoon YG, Freifeld L, Saulnier JL, Riegler C, Engert F, Hughes T, Drobizhev M, Szabo B, Ahrens MB, Flavell SW, Sabatini BL, Boyden ES. Nat Chem Biol. 2018 Feb 26. pii: 10.1038/s41589-018-0004-9. doi: 10.1038/s41589-018-0004-9. 10.1038/s41589-018-0004-9 PubMed 29483642
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