Expression of the Epstein Barr virus transforming protein LMP1 causes a rapid and transient stimulation of the Bcl-2 homologue Mcl-1 levels in B-cell lines
- PMID: 8840972
Expression of the Epstein Barr virus transforming protein LMP1 causes a rapid and transient stimulation of the Bcl-2 homologue Mcl-1 levels in B-cell lines
Abstract
The EBV-encoded latent membrane protein 1 (LMP1) suppresses apoptosis in B lymphocytes through up-regulation of Bcl-2. However, the maximum induction of Bcl-2 by LMP1 takes about 48-72 h. We show in this report that up-regulation of the Bcl-2 homologue Mcl-1 by LMP1 preceded the induction of Bcl-2 and that the up-regulation was transient; therefore, Mcl-1 levels decreased when Bcl-2 levels started to increase. This finding supports the hypothesis that Mcl-1 functions as a rapidly inducible, short-term effector of cell viability. LMP1 also blocked the decline in the Mcl-1 levels in response to apoptotic stimulation triggered by elevated cyclic AMP. This effect of LMP1 was associated with a delayed cell death in the EBV-negative Burkitt lymphoma cell line BL41. The maintenance of Mcl-1 expression by LMP1 is likely to be a crucial immediate-early response that enables cells to survive until Bcl-2 can be up-regulated.
Similar articles
-
The EBV-encoded LMP1 protein inhibits p53-triggered apoptosis but not growth arrest.Oncogene. 1995 Sep 21;11(6):1027-31. Oncogene. 1995. PMID: 7566960
-
The latent membrane protein 1 (LMP1) encoded by Epstein-Barr virus induces expression of the putative oncogene Bcl-3 through activation of the nuclear factor-kappaB.Virus Res. 2008 Feb;131(2):170-9. doi: 10.1016/j.virusres.2007.09.003. Epub 2007 Oct 25. Virus Res. 2008. PMID: 17963943
-
Expression of the Bcl-2 homologue Mcl-1 correlates with survival of peripheral blood B lymphocytes.Cancer Res. 1996 Jan 1;56(1):40-3. Cancer Res. 1996. PMID: 8548771
-
[Intracellular signal transduction pathway and its relation to lymphomagenesis].Nihon Rinsho. 2000 Mar;58(3):529-34. Nihon Rinsho. 2000. PMID: 10741121 Review. Japanese.
-
Mcl-1; the molecular regulation of protein function.FEBS Lett. 2010 Jul 16;584(14):2981-9. doi: 10.1016/j.febslet.2010.05.061. Epub 2010 Jun 11. FEBS Lett. 2010. PMID: 20540941 Review.
Cited by
-
Epstein-Barr virus LMP2A reduces hyperactivation induced by LMP1 to restore normal B cell phenotype in transgenic mice.PLoS Pathog. 2012;8(4):e1002662. doi: 10.1371/journal.ppat.1002662. Epub 2012 Apr 19. PLoS Pathog. 2012. PMID: 22536156 Free PMC article.
-
Hodgkin's disease and the Epstein-Barr virus.Mol Pathol. 2000 Oct;53(5):262-9. doi: 10.1136/mp.53.5.262. Mol Pathol. 2000. PMID: 11091850 Free PMC article. Review.
-
Persistent gamma-herpesvirus infections: what can we learn from an experimental mouse model?J Exp Med. 2002 Apr 1;195(7):F29-32. doi: 10.1084/jem.20020243. J Exp Med. 2002. PMID: 11927639 Free PMC article. Review. No abstract available.
-
The bfl-1 gene is transcriptionally upregulated by the Epstein-Barr virus LMP1, and its expression promotes the survival of a Burkitt's lymphoma cell line.J Virol. 2000 Jul;74(14):6652-8. doi: 10.1128/jvi.74.14.6652-6658.2000. J Virol. 2000. PMID: 10864681 Free PMC article.
-
Expression of the Epstein-Barr virus latent membrane protein 1 induces B cell lymphoma in transgenic mice.Proc Natl Acad Sci U S A. 1998 Sep 29;95(20):11963-8. doi: 10.1073/pnas.95.20.11963. Proc Natl Acad Sci U S A. 1998. PMID: 9751773 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Research Materials