Isolation of an R- M+ mutant of Yersinia enterocolitica serotype O:8 and its application in construction of rough mutants utilizing mini-Tn5 derivatives and lipopolysaccharide-specific phage

doi:10.1128/jb.176.6.1756-1760.1994

. 1994 Mar;176(6):1756-60.

doi: 10.1128/jb.176.6.1756-1760.1994.

Isolation of an R- M+ mutant of Yersinia enterocolitica serotype O:8 and its application in construction of rough mutants utilizing mini-Tn5 derivatives and lipopolysaccharide-specific phage

L Zhang¹, M Skurnik

Affiliations

PMID: 8132471
PMCID: PMC205264
DOI: 10.1128/jb.176.6.1756-1760.1994

Isolation of an R- M+ mutant of Yersinia enterocolitica serotype O:8 and its application in construction of rough mutants utilizing mini-Tn5 derivatives and lipopolysaccharide-specific phage

L Zhang et al. J Bacteriol. 1994 Mar.

. 1994 Mar;176(6):1756-60.

doi: 10.1128/jb.176.6.1756-1760.1994.

Authors

L Zhang¹, M Skurnik

Affiliation

¹ Department of Medical Microbiology, University of Turku, Finland.

PMID: 8132471
PMCID: PMC205264
DOI: 10.1128/jb.176.6.1756-1760.1994

Abstract

A generally applicable procedure was used to isolate a spontaneous restriction-deficient mutant of Yersinia enterocolitica serotype O:8. Transposition frequency in the mutant strain 8081-res was approximately 6.7 x 10(-6) per recipient, while it was practically zero in the wild-type strain 8081-c. Mobilization frequency into 8081-res was 10(5) times higher than that into the wild-type strain. The mutant had lost the ability to express the YenI restriction endonuclease activity present in serotype O:8 strains. This allowed the construction of a transposon library in 8081-res. Insertion mutants with transposons in the genes of the rfa region were selected from this library.

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References

1. Mol Gen Genet. 1980;179(2):421-435 - PubMed
1. Nucleic Acids Res. 1980 Apr 25;8(8):1783-92 - PubMed
1. Eur J Biochem. 1981 Apr;115(3):571-7 - PubMed
1. J Clin Microbiol. 1982 Mar;15(3):491-502 - PubMed
1. FEBS Lett. 1983 Sep 5;161(1):131-4 - PubMed

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[1] Mol Gen Genet. 1980;179(2):421-435 - PubMed

[2] Mol Gen Genet. 1980;179(2):421-435 - PubMed

[3] Nucleic Acids Res. 1980 Apr 25;8(8):1783-92 - PubMed

[4] Nucleic Acids Res. 1980 Apr 25;8(8):1783-92 - PubMed

[5] Eur J Biochem. 1981 Apr;115(3):571-7 - PubMed

[6] Eur J Biochem. 1981 Apr;115(3):571-7 - PubMed

[7] J Clin Microbiol. 1982 Mar;15(3):491-502 - PubMed

[8] J Clin Microbiol. 1982 Mar;15(3):491-502 - PubMed

[9] FEBS Lett. 1983 Sep 5;161(1):131-4 - PubMed

[10] FEBS Lett. 1983 Sep 5;161(1):131-4 - PubMed

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Isolation of an R- M+ mutant of Yersinia enterocolitica serotype O:8 and its application in construction of rough mutants utilizing mini-Tn5 derivatives and lipopolysaccharide-specific phage

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Isolation of an R- M+ mutant of Yersinia enterocolitica serotype O:8 and its application in construction of rough mutants utilizing mini-Tn5 derivatives and lipopolysaccharide-specific phage

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