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. 1994 Sep 14;174(1-2):231-5.
doi: 10.1016/0022-1759(94)90027-2.

Determination of arginase activity in macrophages: a micromethod

I M Corraliza  1 , M L Campo, G Soler, M Modolell
Affiliations

Determination of arginase activity in macrophages: a micromethod

I M Corraliza et al. J Immunol Methods. .

Abstract

We propose a modification of Schimke's method for urea determination as a valuable micromethod for measuring arginase in activated macrophages. The method exhibits the following advantages: (a) it uses small amounts of samples (approximately 25,000 macrophages per assay); (b) it does not interfere with other related metabolites that are also present in the activated macrophage such as citrulline or arginine; (c) saturating concentrations of the substrate arginine can be used; and (d) it is much more sensitive than Schimke's method and can detect small amounts of urea, in the order of 0.02 mumol.

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