Participation of one isozyme of cytosolic glycerophosphate dehydrogenase in the adipose conversion of 3T3 cells
- PMID: 762059
Participation of one isozyme of cytosolic glycerophosphate dehydrogenase in the adipose conversion of 3T3 cells
Abstract
Growing surface cultures of 3T3 cells possess a low level of glycerophosphate dehydrogenase, an important enzyme in triglyceride synthesis. When 3T3-C2, a subline that does not undergo appreciable adipose conversion, reaches confluence, the level of the enzyme does not increase. In 3T3-F442A, a subline that undergoes the conversion with high frequency, the specific activity of the enzyme increases about 600-fold. The enzyme of the adipose 3T3 cells is different from that of non-adipose 3T3 cells in its thermal stability and its affinity for dihydroxyacetone phosphate. The enzyme of the adipose cells probably corresponds to the stable "adult" form of the enzyme, as described previously, and the enzyme of non-adipose 3T3 cells is probably the unstable "embryonic" form. For this reason, the change in the enzyme that takes place during the adipose conversion is greater than would be indicated simply by the total increase in specific activity. If, as seems likely, the two forms of glycerophosphate dehydrogenase are the products of independent genes, the adipose conversion may activate a hitherto silent gene for the stable enzyme.
Similar articles
-
A study of the adipose conversion of suspended 3T3 cells by using glycerophosphate dehydrogenase as differentiation marker.Proc Natl Acad Sci U S A. 1979 Oct;76(10):5138-42. doi: 10.1073/pnas.76.10.5138. Proc Natl Acad Sci U S A. 1979. PMID: 291926 Free PMC article.
-
Increasing activity of enzymes on pathway of triacylglycerol synthesis during adipose conversion of 3T3 cells.J Biol Chem. 1977 Mar 25;252(6):2158-60. J Biol Chem. 1977. PMID: 845168
-
Commitment of adipocyte differentiation in 3T3 cells is inhibited by retinoic acid, and the expression of lipogenic enzymes is modulated through cytoskeleton stabilization.Differentiation. 1987;36(3):211-9. doi: 10.1111/j.1432-0436.1987.tb00195.x. Differentiation. 1987. PMID: 3449400
-
Sensitivity of preadipose 3T3 cells to growth hormone.J Cell Physiol. 1986 Aug;128(2):293-8. doi: 10.1002/jcp.1041280222. J Cell Physiol. 1986. PMID: 3733890
-
[Lipoprotein lipase and adipocyte differentiation].Reprod Nutr Dev (1980). 1985;25(1B):153-8. Reprod Nutr Dev (1980). 1985. PMID: 3887522 Review. French.
Cited by
-
Glucocorticoid regulation of adipocyte differentiation: hormonal triggering of the developmental program and induction of a differentiation-dependent gene.J Cell Biol. 1985 Oct;101(4):1227-35. doi: 10.1083/jcb.101.4.1227. J Cell Biol. 1985. PMID: 3900088 Free PMC article.
-
v-src inhibits differentiation via an extracellular intermediate(s).Mol Cell Biol. 1985 Oct;5(10):2847-50. doi: 10.1128/mcb.5.10.2847-2850.1985. Mol Cell Biol. 1985. PMID: 3939255 Free PMC article.
-
Polychlorinated biphenyl-77 induces adipocyte differentiation and proinflammatory adipokines and promotes obesity and atherosclerosis.Environ Health Perspect. 2008 Jun;116(6):761-8. doi: 10.1289/ehp.10554. Environ Health Perspect. 2008. PMID: 18560532 Free PMC article.
-
Overexpression of hormone-sensitive lipase prevents triglyceride accumulation in adipocytes.J Clin Invest. 1995 Jun;95(6):2652-61. doi: 10.1172/JCI117967. J Clin Invest. 1995. PMID: 7769105 Free PMC article.
-
Lactobacillus plantarum LG42 isolated from gajami sik-hae inhibits adipogenesis in 3T3-L1 adipocyte.Biomed Res Int. 2013;2013:460927. doi: 10.1155/2013/460927. Epub 2013 Feb 28. Biomed Res Int. 2013. PMID: 23555088 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous
