Comparison of virus reactivation, DNA base damage, and cell cycle effects in autologous human melanoma cells resistant to methylating agents
- PMID: 6690060
Comparison of virus reactivation, DNA base damage, and cell cycle effects in autologous human melanoma cells resistant to methylating agents
Abstract
A human melanoma cell line (MM253c1-3D) having an induced stable resistance to the methylating agents 5-(3'-methyl-1-triazeno)imidazole-4-carboxamide, methylnitrosourea, and N-methyl-N'-nitro-N-nitrosoguanidine gave more efficient replication of 5-(3'-methyl-1-triazeno)imidazole-4-carboxamide-treated adenovirus 5 than did the methylation-sensitive parent line (MM253c1). Analysis of DNA hydrolysates from melanoma cells treated with [3H]methylnitrosourea for 1.6 hr showed similar initial levels of 7-methylguanine and 3-methyladenine in both cell lines and substantial excision of the latter lesion after 19 hr. O6-Methylguanine in the DNA of MM253c1 cells also decreased during this period, but in MM253c1-3D cells the initial yield of this lesion was too low for subsequent decrease to be detected. 5-(3'-Methyl-1-triazeno)imidazole-4-carboxamide induced a significant arrest of MM253c1 cells in the G2 phase of the cell cycle. These results show that MM253c1 is a variant of the Mer- phenotype, the resistance of MM253c1-3D cells being attributed to reversion to Mer+ and expressed as very rapid repair of O6-methylguanine lesions.
Similar articles
-
Relationships among cell survival, O6-alkylguanine-DNA alkyltransferase activity, and reactivation of methylated adenovirus 5 and herpes simplex virus type 1 in human melanoma cell lines.Cancer Res. 1989 Sep 1;49(17):4813-7. Cancer Res. 1989. PMID: 2547518
-
Cyclophosphamide resistance developed in a human melanoma cell line.Cancer Treat Rep. 1984 Oct;68(10):1239-46. Cancer Treat Rep. 1984. PMID: 6525597
-
DNA damage induced by a new 2-chloroethyl nitrosourea on malignant melanoma cells.Cancer Res. 1990 Sep 15;50(18):5898-903. Cancer Res. 1990. PMID: 2393861
-
Cross-sensitivity of methylating agents, hydroxyurea, and methotrexate in human tumor cells of the Mer- phenotype.Cancer Res. 1986 Oct;46(10):5009-13. Cancer Res. 1986. PMID: 3756862
-
Kinetics of O6-methylguanine repair in human normal and ataxia telangiectasia cell lines and correlation of repair capacity with cellular sensitivity to methylating agents.Cancer Res. 1981 Dec;41(12 Pt 1):5114-20. Cancer Res. 1981. PMID: 7307010
Cited by
-
Cytotoxicity of 5-(3-methyl-1-triazeno)imidazole-4-carboxamide (MTIC) on Mer+, Mer+Rem- and Mer- cell lines: differential potentiation by 3-acetamidobenzamide.Br J Cancer. 1988 Jan;57(1):54-8. doi: 10.1038/bjc.1988.8. Br J Cancer. 1988. PMID: 2831926 Free PMC article.
-
KSHV infects a subset of human tonsillar B cells, driving proliferation and plasmablast differentiation.J Clin Invest. 2011 Feb;121(2):752-68. doi: 10.1172/JCI44185. Epub 2011 Jan 18. J Clin Invest. 2011. PMID: 21245574 Free PMC article.
-
Host cell reactivation of gamma-irradiated adenovirus 5 in human cell lines of varying radiosensitivity.Br J Cancer. 1992 Jul;66(1):113-8. doi: 10.1038/bjc.1992.226. Br J Cancer. 1992. PMID: 1637659 Free PMC article.
-
Cell cycle perturbation and cell death after exposure of a human lymphoblastoid cell strain to N-methyl-N'-nitro-N-nitrosoguanidine.Am J Pathol. 1989 Jan;134(1):53-61. Am J Pathol. 1989. PMID: 2913827 Free PMC article.
-
Formation and loss of O6-methyldeoxyguanosine in human leucocyte DNA following sequential DTIC and fotemustine chemotherapy.Br J Cancer. 1994 May;69(5):853-7. doi: 10.1038/bjc.1994.165. Br J Cancer. 1994. PMID: 8180013 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Medical
Research Materials
Miscellaneous