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. 1985 Nov 7;83(2):209-15.
doi: 10.1016/0022-1759(85)90242-x.

Detection of eosinophils using an eosinophil peroxidase assay. Its use as an assay for eosinophil differentiation factors

Detection of eosinophils using an eosinophil peroxidase assay. Its use as an assay for eosinophil differentiation factors

M Strath et al. J Immunol Methods. .

Abstract

A colorimetric assay for peroxidase has been applied to the detection of eosinophils in bone marrow cultures and tissue cell suspensions. The substrate solution consists of 0.1 mM o-phenylenediamine in 0.05 M Tris-HCl buffer pH 8.0 containing 0.1% Triton and 1 mM hydrogen peroxide. The method is shown to be an easy and reproducible method of detecting eosinophils, with insignificant interference from neutrophils.

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