Sulfation pattern of chondroitin sulfate in human osteoarthritis cartilages reveals a lower level of chondroitin-4-sulfate

doi:10.1016/j.carbpol.2019.115496

. 2020 Feb 1:229:115496.

doi: 10.1016/j.carbpol.2019.115496. Epub 2019 Oct 21.

Sulfation pattern of chondroitin sulfate in human osteoarthritis cartilages reveals a lower level of chondroitin-4-sulfate

Tzung-Sheng Lin¹, Chang-Hsun Hsieh², Chin Kuo³, Yu-Pu Juang⁴, Yves S Y Hsieh⁵, Hongsen Chiang², Shang-Cheng Hung⁶, Ching-Chuan Jiang², Pi-Hui Liang⁷

Affiliations

¹ School of Pharmacy, College of Medicine, National Taiwan University, Taipei, 100, Taiwan; Genomics Research Center, Academia Sinica, Taipei, 128, Taiwan.
² Department of Orthopedic Surgery, National Taiwan University Hospital, National Taiwan University, Taipei, 100, Taiwan.
³ Department of Radiation Oncology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
⁴ School of Pharmacy, College of Medicine, National Taiwan University, Taipei, 100, Taiwan.
⁵ Division of Glycoscience, Department of Chemistry, School of Engineering Sciences in Chemistry, Biotechnology and Health, Royal Institute of Technology (KTH), Stockholm, SE106 91, Sweden.
⁶ Genomics Research Center, Academia Sinica, Taipei, 128, Taiwan.
⁷ School of Pharmacy, College of Medicine, National Taiwan University, Taipei, 100, Taiwan; Genomics Research Center, Academia Sinica, Taipei, 128, Taiwan. Electronic address: phliang@ntu.edu.tw.

PMID: 31826425
DOI: 10.1016/j.carbpol.2019.115496

Sulfation pattern of chondroitin sulfate in human osteoarthritis cartilages reveals a lower level of chondroitin-4-sulfate

Tzung-Sheng Lin et al. Carbohydr Polym. 2020.

. 2020 Feb 1:229:115496.

doi: 10.1016/j.carbpol.2019.115496. Epub 2019 Oct 21.

Authors

Tzung-Sheng Lin¹, Chang-Hsun Hsieh², Chin Kuo³, Yu-Pu Juang⁴, Yves S Y Hsieh⁵, Hongsen Chiang², Shang-Cheng Hung⁶, Ching-Chuan Jiang², Pi-Hui Liang⁷

Affiliations

¹ School of Pharmacy, College of Medicine, National Taiwan University, Taipei, 100, Taiwan; Genomics Research Center, Academia Sinica, Taipei, 128, Taiwan.
² Department of Orthopedic Surgery, National Taiwan University Hospital, National Taiwan University, Taipei, 100, Taiwan.
³ Department of Radiation Oncology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
⁴ School of Pharmacy, College of Medicine, National Taiwan University, Taipei, 100, Taiwan.
⁵ Division of Glycoscience, Department of Chemistry, School of Engineering Sciences in Chemistry, Biotechnology and Health, Royal Institute of Technology (KTH), Stockholm, SE106 91, Sweden.
⁶ Genomics Research Center, Academia Sinica, Taipei, 128, Taiwan.
⁷ School of Pharmacy, College of Medicine, National Taiwan University, Taipei, 100, Taiwan; Genomics Research Center, Academia Sinica, Taipei, 128, Taiwan. Electronic address: phliang@ntu.edu.tw.

PMID: 31826425
DOI: 10.1016/j.carbpol.2019.115496

Abstract

Chondroitin sulfates (CS) account for more than 80% of the glycosaminoglycans of articular cartilage, which impart its physiological functions. We quantified the absolute concentration of the CS components of the full thickness cartilages from the knees of patients with terminal-phase osteoarthritis. Osteochondrol biopsies were removed from the medial femoral condyle and lateral femoral condyle of sixty female patients received total knee arthroplasty, aged from 58 to 83 years old. We found the total CS concentrations and chondroitin-4-sulfate disaccharide were significantly lowered in osteoarthritic samples. Microstructure analysis indicated while chondroitin-0-sulfate was equally distributed across different zones of the osteoarthritic cartilages, chondroitin-4-sulfate is significantly less in the deep zones. Down-regulation of sulfotransferases, the enzymes responsible for CS sulfation, in the lesion site of cartilage were observed. Our study suggested chondroitin-4-sulfate down-regulation can be a diagnostic marker for degraded osteoarthritis cartilage, with potential implications in cartilage regeneration.

Keywords: Adult cartilage; Chondroitin sulfate; Osteoarthritis; Sulfatase; Sulfotransferase.

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Sulfation pattern of chondroitin sulfate in human osteoarthritis cartilages reveals a lower level of chondroitin-4-sulfate

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Sulfation pattern of chondroitin sulfate in human osteoarthritis cartilages reveals a lower level of chondroitin-4-sulfate

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