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. 2018 Mar:99:184-190.
doi: 10.1016/j.biopha.2018.01.041.

Baicalin alleviates IL-1β-induced inflammatory injury via down-regulating miR-126 in chondrocytes

Xirui Yang  1 , Qi Zhang  1 , Zhaomeng Gao  2 , Chunyan Yu  1 , Lei Zhang  1
Affiliations

Baicalin alleviates IL-1β-induced inflammatory injury via down-regulating miR-126 in chondrocytes

Xirui Yang et al. Biomed Pharmacother. 2018 Mar.

Expression of concern in

Abstract

Baicalin is a flavonoid extracted from Scutellaria baicalensis Georgi, with anti-inflammatory and anti-apoptotic activities. The objective of this study was to explore the effect and mechanism of baicalin on chondrocyte inflammatory response in OA. Different concentrations of IL-1β (0, 0.1, 2, 5 and 10 ng/mL) were used to simulate inflammatory injury in CHON-001 cells. The expression of miR-126 was altered by transfection with miR-126 mimic. Thereafter, cells were treated with baicalin, and cell viability, apoptosis, the expressions of apoptosis-related protein and pro-inflammatory factors were respectively detected using CCK-8 assay, flow cytometry, qRT-PCR and western blot analysis. We found that IL-1β induced a significantly inflammatory injury in CHON-001 cells. Baicalin alleviated IL-1β-induced inflammatory injury, as it increased cell viability, decreased cell apoptosis and repressed the production of IL-6, IL-8 and TNF-α. miR-126 was up-regulated by IL-1β treatment while was down-regulated by baicalin. More interestingly, the protective actions of baicalin on IL-1β-injured CHON-001 cells were partially eliminated by miR-126 overexpression. Further, NF-κB signaling pathway was activated by IL-1β, and deactivated by addition of baicalin. The deactivation of NF-κB signaling pathway induced by baicalin upon IL-1β exposure was recovered by miR-126 overexpression. In conclusion, this study demonstrated that baicalin protected CHON-001 cells against IL-1β-induced inflammatory injury possibly via down-regulation of miR-126 and thereby deactivation of NF-κB signaling pathway.

Keywords: Baicalin; Inflammation injury; NF-κB signaling pathway; Osteoarthritis; miR-126.

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