Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017 Oct 5;68(1):144-157.e5.
doi: 10.1016/j.molcel.2017.09.003. Epub 2017 Sep 28.

P-Body Purification Reveals the Condensation of Repressed mRNA Regulons

Arnaud Hubstenberger  1 , Maïté Courel  2 , Marianne Bénard  2 , Sylvie Souquere  3 , Michèle Ernoult-Lange  2 , Racha Chouaib  4 , Zhou Yi  2 , Jean-Baptiste Morlot  5 , Annie Munier  6 , Magali Fradet  7 , Maëlle Daunesse  8 , Edouard Bertrand  9 , Gérard Pierron  3 , Julien Mozziconacci  5 , Michel Kress  2 , Dominique Weil  10
Affiliations
Free article

P-Body Purification Reveals the Condensation of Repressed mRNA Regulons

Arnaud Hubstenberger et al. Mol Cell. .
Free article

Abstract

Within cells, soluble RNPs can switch states to coassemble and condense into liquid or solid bodies. Although these phase transitions have been reconstituted in vitro, for endogenous bodies the diversity of the components, the specificity of the interaction networks, and the function of the coassemblies remain to be characterized. Here, by developing a fluorescence-activated particle sorting (FAPS) method to purify cytosolic processing bodies (P-bodies) from human epithelial cells, we identified hundreds of proteins and thousands of mRNAs that structure a dense network of interactions, separating P-body from non-P-body RNPs. mRNAs segregating into P-bodies are translationally repressed, but not decayed, and this repression explains part of the poor genome-wide correlation between RNA and protein abundance. P-bodies condense thousands of mRNAs that strikingly encode regulatory processes. Thus, we uncovered how P-bodies, by condensing and segregating repressed mRNAs, provide a physical substrate for the coordinated regulation of posttranscriptional mRNA regulons.

Keywords: P-bodies; RNP condensation; RNP granules; decay; gene expression regulation; phase separation; phase transition; regulon; stress granules; translation repression.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

MeSH terms

LinkOut - more resources