Osthole attenuates lipid accumulation, regulates the expression of inflammatory mediators, and increases antioxidants in FL83B cells

doi:10.1016/j.biopha.2017.04.051

. 2017 Jul:91:78-87.

doi: 10.1016/j.biopha.2017.04.051. Epub 2017 May 23.

Osthole attenuates lipid accumulation, regulates the expression of inflammatory mediators, and increases antioxidants in FL83B cells

Wen-Chung Huang¹, Po-Chen Liao², Chun-Hsun Huang³, Sindy Hu⁴, Shih-Chun Huang⁵, Shu-Ju Wu⁶

Affiliations

¹ Graduate Institute of Health Industry Technology, Research Center for Industry of Human Ecology, Research Center for Chinese Herbal Medicine, College of Human Ecology, Chang Gung University of Science and Technology, No.261, Wenhua 1st Rd., Guishan Dist., Taoyuan City 33303, Taiwan; Department of Nutrition and Health Sciences, Research Center for Food and Cosmetic Safety, and Research Center for Chinese Herbal Medicine, College of Human Ecology, Chang Gung University of Science and Technology, No.261, Wenhua 1st Rd., Guishan Dist., Taoyuan City 33303, Taiwan.
² Department of Nutrition and Health Sciences, Research Center for Food and Cosmetic Safety, and Research Center for Chinese Herbal Medicine, College of Human Ecology, Chang Gung University of Science and Technology, No.261, Wenhua 1st Rd., Guishan Dist., Taoyuan City 33303, Taiwan; Institute of Oral Biology, National Yang-Ming University, Taipei, Taiwan.
³ Department of Cosmetic Science, Chang Gung University of Science and Technology, No.261, Wenhua 1st Rd., Guishan Dist., Taoyuan City 33303, Taiwan; Aesthetic Medical Center, Department of Dermatology, Chang Gung Memorial Hospital, Guishan Dist., Taoyuan 33303, Taiwan.
⁴ Aesthetic Medical Center, Department of Dermatology, Chang Gung Memorial Hospital, Guishan Dist., Taoyuan 33303, Taiwan.
⁵ Department of Nutrition and Health Sciences, Research Center for Food and Cosmetic Safety, and Research Center for Chinese Herbal Medicine, College of Human Ecology, Chang Gung University of Science and Technology, No.261, Wenhua 1st Rd., Guishan Dist., Taoyuan City 33303, Taiwan.
⁶ Department of Nutrition and Health Sciences, Research Center for Food and Cosmetic Safety, and Research Center for Chinese Herbal Medicine, College of Human Ecology, Chang Gung University of Science and Technology, No.261, Wenhua 1st Rd., Guishan Dist., Taoyuan City 33303, Taiwan; Aesthetic Medical Center, Department of Dermatology, Chang Gung Memorial Hospital, Guishan Dist., Taoyuan 33303, Taiwan. Electronic address: sjwu@gw.cgust.edu.tw.

PMID: 28448873
DOI: 10.1016/j.biopha.2017.04.051

Osthole attenuates lipid accumulation, regulates the expression of inflammatory mediators, and increases antioxidants in FL83B cells

Wen-Chung Huang et al. Biomed Pharmacother. 2017 Jul.

. 2017 Jul:91:78-87.

doi: 10.1016/j.biopha.2017.04.051. Epub 2017 May 23.

Authors

Wen-Chung Huang¹, Po-Chen Liao², Chun-Hsun Huang³, Sindy Hu⁴, Shih-Chun Huang⁵, Shu-Ju Wu⁶

Affiliations

¹ Graduate Institute of Health Industry Technology, Research Center for Industry of Human Ecology, Research Center for Chinese Herbal Medicine, College of Human Ecology, Chang Gung University of Science and Technology, No.261, Wenhua 1st Rd., Guishan Dist., Taoyuan City 33303, Taiwan; Department of Nutrition and Health Sciences, Research Center for Food and Cosmetic Safety, and Research Center for Chinese Herbal Medicine, College of Human Ecology, Chang Gung University of Science and Technology, No.261, Wenhua 1st Rd., Guishan Dist., Taoyuan City 33303, Taiwan.
² Department of Nutrition and Health Sciences, Research Center for Food and Cosmetic Safety, and Research Center for Chinese Herbal Medicine, College of Human Ecology, Chang Gung University of Science and Technology, No.261, Wenhua 1st Rd., Guishan Dist., Taoyuan City 33303, Taiwan; Institute of Oral Biology, National Yang-Ming University, Taipei, Taiwan.
³ Department of Cosmetic Science, Chang Gung University of Science and Technology, No.261, Wenhua 1st Rd., Guishan Dist., Taoyuan City 33303, Taiwan; Aesthetic Medical Center, Department of Dermatology, Chang Gung Memorial Hospital, Guishan Dist., Taoyuan 33303, Taiwan.
⁴ Aesthetic Medical Center, Department of Dermatology, Chang Gung Memorial Hospital, Guishan Dist., Taoyuan 33303, Taiwan.
⁵ Department of Nutrition and Health Sciences, Research Center for Food and Cosmetic Safety, and Research Center for Chinese Herbal Medicine, College of Human Ecology, Chang Gung University of Science and Technology, No.261, Wenhua 1st Rd., Guishan Dist., Taoyuan City 33303, Taiwan.
⁶ Department of Nutrition and Health Sciences, Research Center for Food and Cosmetic Safety, and Research Center for Chinese Herbal Medicine, College of Human Ecology, Chang Gung University of Science and Technology, No.261, Wenhua 1st Rd., Guishan Dist., Taoyuan City 33303, Taiwan; Aesthetic Medical Center, Department of Dermatology, Chang Gung Memorial Hospital, Guishan Dist., Taoyuan 33303, Taiwan. Electronic address: sjwu@gw.cgust.edu.tw.

PMID: 28448873
DOI: 10.1016/j.biopha.2017.04.051

Abstract

Osthole is found in Cnidium monnieri (L.) and has anti-inflammatory and anti-oxidative properties. It also inhibits the proliferation of hepatocellular carcinoma cells. This study aimed to evaluate the osthole suppressive nonalcoholic fatty liver disease effects in oleic acid (OA)-induced hepatic steatosis and if it can modulate inflammatory responses and oxidative stress. FL83B cells were pretreated with OA (250μΜ) for 24h, and then added different concentrations of osthole (3-100μM) for 24h. Subsequently, lipolysis and transcription factors of adipogenesis and phosphorylation of AMP-activated protein kinase proteins were measured. In addition, cells with OA-induced steatosis were H₂O₂-stimulated, and then incubated with osthole to evaluated if it could suppress its progression to steatohepatitis. Osthole significantly enhanced glycerol release and lipolysis protein expression. Osthole also promoted phosphorylation of AMP-activated protein kinases and increased the activity of triglyceride lipase and hormone- sensitive lipase. Osthole suppressed the nuclear transcription factor kappa-B and the p38 mitogen-activated protein kinase pathway, and decreased the malondialdehyde concentration in FL83B cells with OA-induced steatosis that were treated with H₂O₂. These results suggest that osthole might suppress nonalcoholic fatty liver disease by decreasing lipid accumulation, and through its anti-oxidative and anti-inflammatory effects via blocked NF-κB and MAPK signaling pathways.

Keywords: Antioxidant; NF-κB; Nonalcoholic fatty liver disease; Osthole; p38 MAPK.

PubMed Disclaimer

Cited by

The combined usage of Matrine and Osthole inhibited endoplasmic reticulum apoptosis induced by PCV2.
Xu Y, Sun P, Wan S, Guo J, Zheng X, Sun Y, Fan K, Yin W, Sun N, Li H. Xu Y, et al. BMC Microbiol. 2020 Oct 12;20(1):303. doi: 10.1186/s12866-020-01986-2. BMC Microbiol. 2020. PMID: 33046006 Free PMC article.
Osthole attenuates myocardial ischemia/reperfusion injury in rats by inhibiting apoptosis and inflammation.
Wu J, Yang Y, Xun N, Zeng L, Li Z, Yang W, Liang Y, Ma Z, Tang H. Wu J, et al. Am J Transl Res. 2018 Apr 15;10(4):1109-1116. eCollection 2018. Am J Transl Res. 2018. PMID: 29736204 Free PMC article.
The Effect of Osthole on Transient Receptor Potential Channels: A Possible Alternative Therapy for Atopic Dermatitis.
Kordulewska NK, Król-Grzymała A. Kordulewska NK, et al. J Inflamm Res. 2024 Feb 9;17:881-898. doi: 10.2147/JIR.S425978. eCollection 2024. J Inflamm Res. 2024. PMID: 38351985 Free PMC article.
Identification and Comparison of Constituents of Aurantii Fructus and Aurantii Fructus Immaturus by UFLC-DAD-Triple TOF-MS/MS.
Bai Y, Zheng Y, Pang W, Peng W, Wu H, Yao H, Li P, Deng W, Cheng J, Su W. Bai Y, et al. Molecules. 2018 Mar 30;23(4):803. doi: 10.3390/molecules23040803. Molecules. 2018. PMID: 29601542 Free PMC article.
Osthol Ameliorates Kidney Damage and Metabolic Syndrome Induced by a High-Fat/High-Sugar Diet.
García-Arroyo FE, Gonzaga-Sánchez G, Tapia E, Muñoz-Jiménez I, Manterola-Romero L, Osorio-Alonso H, Arellano-Buendía AS, Pedraza-Chaverri J, Roncal-Jiménez CA, Lanaspa MA, Johnson RJ, Sánchez-Lozada LG. García-Arroyo FE, et al. Int J Mol Sci. 2021 Feb 28;22(5):2431. doi: 10.3390/ijms22052431. Int J Mol Sci. 2021. PMID: 33670975 Free PMC article.

See all "Cited by" articles

MeSH terms

Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions

Substances

Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions

LinkOut - more resources

Full Text Sources
- ClinicalKey
- Elsevier Science
Other Literature Sources
- scite Smart Citations
Medical
- MedlinePlus Health Information

Save citation to file

Email citation

Add to Collections

Add to My Bibliography

Your saved search

Create a file for external citation management software

Your RSS Feed

Osthole attenuates lipid accumulation, regulates the expression of inflammatory mediators, and increases antioxidants in FL83B cells

Affiliations

Osthole attenuates lipid accumulation, regulates the expression of inflammatory mediators, and increases antioxidants in FL83B cells

Authors

Affiliations

Abstract

Similar articles

Cited by

MeSH terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources

Medical

Abstract

Similar articles

Cited by

MeSH terms

Substances

Related information

LinkOut - more resources

Full Text Sources

Other Literature Sources

Medical