Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 2017 Feb 21;12(2):e0172214.
doi: 10.1371/journal.pone.0172214. eCollection 2017.

Differentially expressed proteins underlying childhood cortical dysplasia with epilepsy identified by iTRAQ proteomic profiling

Lu Qin  1 , Xi Liu  1 , Shiyong Liu  2 , Yi Liu  1 , Yixuan Yang  3 , Hui Yang  2 , Yangmei Chen  1 , Lifen Chen  1
Affiliations
Comparative Study

Differentially expressed proteins underlying childhood cortical dysplasia with epilepsy identified by iTRAQ proteomic profiling

Lu Qin et al. PLoS One. .

Abstract

Cortical dysplasia accounts for at least 14% of epilepsy cases, and is mostly seen in children. However, the understanding of molecular mechanisms and pathogenesis underlying cortical dysplasia is limited. The aim of this cross-sectional study is to identify potential key molecules in the mechanisms of cortical dysplasia by screening the proteins expressed in brain tissues of childhood cortical dysplasia patients with epilepsy using isobaric tags for relative and absolute quantitation-based tandem mass spectrometry compared to controls, and several differentially expressed proteins that are not reported to be associated with cortical dysplasia previously were selected for validation using real-time polymerase chain reaction, immunoblotting and immunohistochemistry. 153 out of 3340 proteins were identified differentially expressed between childhood cortical dysplasia patients and controls. And FSCN1, CRMP1, NDRG1, DPYSL5, MAP4, and FABP3 were selected for validation and identified to be increased in childhood cortical dysplasia patients, while PRDX6 and PSAP were identified decreased. This is the first report on differentially expressed proteins in childhood cortical dysplasia. We identified differential expression of FSCN1, CRMP1, NDRG1, DPYSL5, MAP4, FABP3, PRDX6 and PSAP in childhood cortical dysplasia patients, these proteins are involved in various processes and have various function. These results may provide new directions or targets for the research of childhood cortical dysplasia, and may be helpful in revealing molecular mechanisms and pathogenesis and/or pathophysiology of childhood cortical dysplasia if further investigated.

PubMed Disclaimer

Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. Molecule functional categories of 153 differentially exprssed proteins using the PANTHER Classification System.
Fig 2
Fig 2. Relative mRNA expression levels of NDRG1, FSCN1, FABP3, DPYSL5, PSAP, MAP4, CRMP1, PRDX6.
*p<0.05 compared to controls.
Fig 3
Fig 3. Immunoblotting for CRMP1, DPYSL5, NDRG1, FSCN1 and PRDX6.
Quantification of protein levels showed increased expression of CRMP1 (A), DPYSL5 (B), NDRG1 (C) and FSCN1 (D) and decreased expression of PRDX6 (E) in brain tissue of childhood cortical dysplasia patients compared to controls. *p<0.05 compared to controls.
Fig 4
Fig 4. Immunohistochemistry of CRMP1, DPYSL5 and FSCN1.
IHC score of CRMP1 (A & B), DPYSL5 (C & D) and FSCN1 (E & F) were significantly increased in brain tissues of childhood cortical dysplasia patients with epilepsy compared to controls. *p<0.05 compared to controls.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Crino PB, Chou K. Epilepsy and Cortical Dysplasias. Curr Treat Options Neurol. 2000; 2: 543–552. - PubMed
    1. Sisodiya SM. Malformations of cortical development: burdens and insights from important causes of human epilepsy. Lancet Neurol. 2004; 3: 29–38. - PubMed
    1. Lee SK, Kim DW. Focal cortical dysplasia and epilepsy surgery. J Epilepsy Res. 2013; 3:43–47. 10.14581/jer.13009 - DOI - PMC - PubMed
    1. Shaker T, Bernier A, Carmant L. Focal Cortical Dysplasia in Childhood Epilepsy. Semin Pediatr Neurol. 2016. May;23(2):108–19. 10.1016/j.spen.2016.06.007 - DOI - PubMed
    1. Mériaux C, Franck J, Park DB, Quanico J, Kim YH, Chung CK, et al. Human temporal lobe epilepsy analyses by tissue proteomics. Hippocampus. 2014. June;24(6):628–42. 10.1002/hipo.22246 - DOI - PubMed

MeSH terms

Substances

Supplementary concepts

Grants and funding

This study is supported by National Natural Science Foundation of China (grant number: 81571259, website of funder: http://www.nsfc.gov.cn/, receiver: YC), and Chongqing Municipal Public Health Bureau, Chongqing People's Municipal Government (grant number: 2015ZDXM011, website of funder: http://www.cqwsjsw.gov.cn/, receiver: LC). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.