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. 2016 Feb 19:16:12.
doi: 10.1186/s12935-016-0285-6. eCollection 2016.

MicroRNA-410-5p as a potential serum biomarker for the diagnosis of prostate cancer

Jiaqi Wang  1 , Huamao Ye  2 , Dandan Zhang  1 , Yijun Hu  1 , Xiya Yu  3 , Long Wang  1 , Changjing Zuo  4 , Yongwei Yu  5 , Guixia Xu  1 , Shanrong Liu  1
Affiliations

MicroRNA-410-5p as a potential serum biomarker for the diagnosis of prostate cancer

Jiaqi Wang et al. Cancer Cell Int. .

Abstract

Background: Prostate cancer (PCa) remains to be a diagnostic challenge due to its variable presentation and the lack of reliable diagnosis tool. MicroRNAs (miRNAs) regulate gene in extensive range of pathophysiologic processes. Plasma miRNAs are ideal biomarkers in heart failure, diabetes and other disease. However, using circulating miRNAs as biomarkers for the diagnosis of PCa is still unknown.

Methods: 149 PCa patients, 57 healthy controls, and 121 non-cancer patients (benign prostatic hyperplasia and other urinary diseases) were enrolled in this study. The reverse transcription of miRNA and SYBR-Green-based double standards curve miRNA quantitative polymerase chain reactions (qPCR) were used to evaluate the dysregulated miR-410-5p. Receiver operator characteristic (ROC) curve analysis was used to evaluate the diagnostic accuracy of miR-410-5p identified as the alternative biomarker.

Results: Circulating miRNA-410-5p (miR-410-5p) level was significantly higher in the PCa patients than in healthy controls or non-cancer patients. ROC curve analysis showed that plasma miR-410-5p was a specific diagnostic biomarker of PCa with an area under curve(AUC) of 0.8097 (95 % confidence interval, 0.7371-0.8823; P < 0.001).

Conclusions: The serum miR-410-5p level is a potential biomarker for the diagnosis of PCa.

Keywords: Circulating miRNA; Diagnosis; Prostate cancer; miR-410-5p.

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Figures

Fig. 1
Fig. 1
Serum miR-410-5p levels in different groups. a The relative serum miR-410-5p levels in 20 healthy controls and 51 PCa patients. The value of miR-410-5p was provided above the histogram. **, P < 0.01. b The Serum miR-410-5p level in 57 healthy volunteers, 121 non-PCA controls (include 81 patients with BPH and 40 patients with other urinary diseases) and 149 PCA patients. The value of miR-410-5p was provided above the histogram. **, P < 0.01. c The Serum miR-410 levels in 65 low-risk PCa patients (Gleason level <7) and 84 high-intermediate-risk PCa (Gleason level >=7). The value of miR-410-5p was provided above the histogram. **, P < 0.01
Fig. 2
Fig. 2
Serum miR-410-5p levels in PCa patients and comparison test with miR-1228 and let-7c. a The relative serum miR-410-5p level in 115 good prognosis patient (no recurrence) and 34 PCa patients with poor prognosis. The expression levels of miR-410-5p was normalized by U6. The value of miR-410-5p was provided above the histogram. **, P < 0.01. b The relative serum level of let-7c, miR-410-5p, and miR1228 in 73 samples (Including 34 randomly selected PCa patients, 25 BPH patients and 14 healthy controls). The expression levels were normalized by U6. The value of miRNAs level and p value were provided above the histogram
Fig. 3
Fig. 3
ROC curve test of serum miR-410-5p. a ROC curve of miR-410-5p, PSA and both for all patients with PCa versus all controls in 327 samples. The optimum cutoff for miR-410-5p expression and PSA was 21.4 copy/ml and 4 pg/ml, respectively for diagnosis for PCa. b ROC curve of miR-410-5p, PSA and both for low-risk PCa (Gleason score <7) versus high-intermediate-risk PCa (Gleason score >=7) in 149 PCa patients. The optimum cutoff for miR-410-5p expression and PSA was 64.1 copy/ml and 13.12 ng/L, respectively
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