Quantitative Investigation on Correlation Between IgG and FcRn During Gestation and Lactating Periods in Rat
- PMID: 26699647
- DOI: 10.1111/aji.12465
Quantitative Investigation on Correlation Between IgG and FcRn During Gestation and Lactating Periods in Rat
Abstract
Problem: IgG is transformed from maternal serum into the offspring as passive immunization by FcRn expressed in placenta and/or infant intestine. This study aimed to investigate the quantitative correlation between IgG and FcRn during gestation and lactating periods in rat.
Method of study: ELISA was performed to determine the variation of maternal and offspring IgG. Western blot and RT-qPCR were carried out to characterize FcRn expression in placenta and infant intestine.
Results: Maternal serum IgG appeared higher in first 2 weeks of lactation. The embryo IgG was in accordance with the FcRn expression in placenta. During the post-natal, the serum IgG concentration in feta was obviously lower than maternal IgG on day 0 before uptaking colostrums and rapidly increased on day 1.
Conclusion: These findings would provide clues for the endogenous transportation and exogenous administration of IgG for a better IgG intervention in offspring.
Keywords: FcRn; immunoglobulin (IgG); intestinal transfer; passive immunization; placental transfer.
© 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
Similar articles
-
The transfer of maternal antigen-specific IgG regulates the development of allergic airway inflammation early in life in an FcRn-dependent manner.Biochem Biophys Res Commun. 2010 Apr 30;395(2):238-43. doi: 10.1016/j.bbrc.2010.03.170. Epub 2010 Apr 1. Biochem Biophys Res Commun. 2010. PMID: 20362552 Free PMC article.
-
Maternal-Foetal Diabetes Modifies Neonatal Fc Receptor Expression on Human Leucocytes.Scand J Immunol. 2016 Oct;84(4):237-44. doi: 10.1111/sji.12466. Scand J Immunol. 2016. PMID: 27469170
-
Neonatal Fc receptor (FcRn): a novel target for therapeutic antibodies and antibody engineering.J Drug Target. 2014 May;22(4):269-78. doi: 10.3109/1061186X.2013.875030. Epub 2014 Jan 9. J Drug Target. 2014. PMID: 24404896 Review.
-
Qualification of a homogeneous cell-based neonatal Fc receptor (FcRn) binding assay and its application to studies on Fc functionality of IgG-based therapeutics.J Immunol Methods. 2013 Apr 30;390(1-2):81-91. doi: 10.1016/j.jim.2013.01.011. Epub 2013 Feb 4. J Immunol Methods. 2013. PMID: 23384837
-
New functions of the MHC class I-related Fc receptor, FcRn.Biochem Soc Trans. 1997 May;25(2):481-6. doi: 10.1042/bst0250481. Biochem Soc Trans. 1997. PMID: 9191140 Review.
Cited by
-
Quantitative mechanistic model reveals key determinants of placental IgG transfer and informs prenatal immunization strategies.PLoS Comput Biol. 2023 Nov 7;19(11):e1011109. doi: 10.1371/journal.pcbi.1011109. eCollection 2023 Nov. PLoS Comput Biol. 2023. PMID: 37934786 Free PMC article.
-
Opportunities and Challenges for PBPK Model of mAbs in Paediatrics and Pregnancy.AAPS J. 2022 Jun 1;24(4):72. doi: 10.1208/s12248-022-00722-0. AAPS J. 2022. PMID: 35650328 Review.
-
Rat Milk and Plasma Immunological Profile throughout Lactation.Nutrients. 2021 Apr 11;13(4):1257. doi: 10.3390/nu13041257. Nutrients. 2021. PMID: 33920419 Free PMC article.
-
In Translation: FcRn across the Therapeutic Spectrum.Int J Mol Sci. 2021 Mar 17;22(6):3048. doi: 10.3390/ijms22063048. Int J Mol Sci. 2021. PMID: 33802650 Free PMC article. Review.
-
Lactobacillus fermentum CECT5716 Supplementation in Rats during Pregnancy and Lactation Impacts Maternal and Offspring Lipid Profile, Immune System and Microbiota.Cells. 2020 Feb 28;9(3):575. doi: 10.3390/cells9030575. Cells. 2020. PMID: 32121244 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
