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. 2015 Apr 3;348(6230):120-3.
doi: 10.1126/science.aaa2618.

Plant biology. Suppression of endogenous gene silencing by bidirectional cytoplasmic RNA decay in Arabidopsis

Xinyan Zhang  1 , Ying Zhu  1 , Xiaodan Liu  1 , Xinyu Hong  1 , Yang Xu  2 , Ping Zhu  2 , Yang Shen  3 , Huihui Wu  1 , Yusi Ji  1 , Xing Wen  1 , Chen Zhang  1 , Qiong Zhao  1 , Yichuan Wang  1 , Jian Lu  4 , Hongwei Guo  5
Affiliations

Plant biology. Suppression of endogenous gene silencing by bidirectional cytoplasmic RNA decay in Arabidopsis

Xinyan Zhang et al. Science. .

Abstract

Plant immunity against foreign gene invasion takes advantage of posttranscriptional gene silencing (PTGS). How plants elaborately avert inappropriate PTGS of endogenous coding genes remains unclear. We demonstrate in Arabidopsis that both 5'-3' and 3'-5' cytoplasmic RNA decay pathways act as repressors of transgene and endogenous PTGS. Disruption of bidirectional cytoplasmic RNA decay leads to pleiotropic developmental defects and drastic transcriptomic alterations, which are substantially rescued by PTGS mutants. Upon dysfunction of bidirectional RNA decay, a large number of 21- to 22-nucleotide endogenous small interfering RNAs are produced from coding transcripts, including multiple microRNA targets, which could interfere with their cognate gene expression and functions. This study highlights the risk of unwanted PTGS and identifies cytoplasmic RNA decay pathways as safeguards of plant transcriptome and development.

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