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. 2014 Jan;20(1):153-9.
doi: 10.1007/s12253-013-9678-1. Epub 2013 Sep 13.

MicroRNA expression profiles in Kaposi's sarcoma

Ana Maria Catrina Ene  1 , Ioana Borze, Mohamed Guled, Mariana Costache, Gayle Leen, Maria Sajin, Elena Ionica, Aura Chitu, Sakari Knuutila
Affiliations
Free article

MicroRNA expression profiles in Kaposi's sarcoma

Ana Maria Catrina Ene et al. Pathol Oncol Res. 2014 Jan.
Free article

Abstract

Kaposi's sarcoma (KS) is a mesenchymal tumor, caused by Human herpesvirus 8 (HHV8) with molecular and cytogenetic changes poorly understood. To gain further insight on the underlying molecular changes in KS, we performed microRNA (miRNA) microarray analysis of 17 Kaposi's sarcoma specimens. Three normal skin specimens were used as controls. The most significant differentially expressed miRNA were confirmed by quantitative reverse transcriptase polymerase chain reaction (RT-PCR). We detected in KS versus normal skin 185 differentially expressed miRNAs, 76 were upregulated and 109 were downregulated. The most significantly downregulated miRNAs were miR-99a, miR-200 family, miR-199b-5p, miR-100 and miR-335, whereas kshv-miR-K12-4-3p, kshv-miR-K12-1, kshv-miR-K12-2, kshv-miR-K12-4-5p and kshv-miR-K12-8 were significantly upregulated. High expression levels of kshv-miR-K12-1 (p = 0.004) and kshv-miR-K12-4-3p (p = 0.001) was confirmed by RT-PCR. The predicted target genes for differentially expressed miRNAs included genes which are involved in a variety of cellular processes such as angiogenesis (i.e. THBS1) and apoptosis (i.e. CASP3, MCL1), suggesting a role for these miRNAs in Kaposi's sarcoma pathogenesis.

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References

    1. Nat Genet. 2009 Jan;41(1):130-4 - PubMed
    1. N Engl J Med. 2000 Apr 6;342(14):1027-38 - PubMed
    1. Dev Biol. 2007 Feb 1;302(1):1-12 - PubMed
    1. Leuk Res. 2011 Feb;35(2):188-95 - PubMed
    1. Philos Trans R Soc Lond B Biol Sci. 2001 Apr 29;356(1408):499-516 - PubMed

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