Lactobacillus gasseri SF1183 affects intestinal epithelial cell survival and growth
- PMID: 23894414
- PMCID: PMC3720908
- DOI: 10.1371/journal.pone.0069102
Lactobacillus gasseri SF1183 affects intestinal epithelial cell survival and growth
Retraction in
-
Retraction: Lactobacillus gasseri SF1183 Affects Intestinal Epithelial Cell Survival and Growth.PLoS One. 2020 Mar 17;15(3):e0230791. doi: 10.1371/journal.pone.0230791. eCollection 2020. PLoS One. 2020. PMID: 32182284 Free PMC article. No abstract available.
Abstract
It is now commonly accepted that the intestinal microbiota plays a crucial role in the gut physiology and homeostasis, and that both qualitative and quantitative alterations in the compositions of the gut flora exert profound effects on the host's intestinal cells. In spite of this, the details of the interaction between commensal bacteria and intestinal cells are still largely unknown and only in few cases the molecular mechanisms have been elucidated. Here we analyze the effects of molecules produced and secreted by Lactobacillus gasseri SF1183 on human intestinal HCT116 cells. L. gasseri is a well known species of lactic acid bacteria, commonly associated to the human intestine and SF1183 is a human strain previously isolated from an ileal biopsy of an healthy volunteer. SF1183 produces and secretes, in a growth phase-dependent way, molecule(s) able to drastically interfere with HCT116 cell proliferation. Although several attempts to purify and identify the bioactive molecule(s) have been so far unsuccessful, a partial characterization has indicated that it is smaller than 3 kDa, thermostable and of proteinaceous nature. L. gasseri molecule(s) stimulate a G1-phase arrest of the cell cycle by up-regulation of p21WAF1 rendering cells protected from intrinsic and extrinsic apoptosis. A L. gasseri-mediated reduction of apoptosis and of cell proliferation could be relevant in protecting epithelial barrier integrity and helping in reconstituting tissutal homeostasis.
Conflict of interest statement
Figures
Similar articles
-
Modulation of intestinal epithelial cell proliferation and apoptosis by Lactobacillus gasseri SF1183.Sci Rep. 2022 Nov 24;12(1):20248. doi: 10.1038/s41598-022-24483-0. Sci Rep. 2022. PMID: 36424419 Free PMC article.
-
Characterization of intestinal bacteria tightly bound to the human ileal epithelium.Res Microbiol. 2009 Dec;160(10):817-23. doi: 10.1016/j.resmic.2009.09.009. Epub 2009 Sep 24. Res Microbiol. 2009. PMID: 19782749
-
Lactobacillus casei NY1301 increases the adhesion of Lactobacillus gasseri NY0509 to human intestinal caco-2 cells.Biosci Biotechnol Biochem. 2001 Oct;65(10):2326-9. doi: 10.1271/bbb.65.2326. Biosci Biotechnol Biochem. 2001. PMID: 11758933
-
[Biological properties of Lactobacillus surface proteins].Postepy Hig Med Dosw (Online). 2013 Apr 4;67:229-37. doi: 10.5604/17322693.1043393. Postepy Hig Med Dosw (Online). 2013. PMID: 23619222 Review. Polish.
-
Interactions between the microbiota and the intestinal mucosa.Eur J Clin Nutr. 2002 Aug;56 Suppl 3:S60-4. doi: 10.1038/sj.ejcn.1601489. Eur J Clin Nutr. 2002. PMID: 12142966 Review.
Cited by
-
Retraction: Lactobacillus gasseri SF1183 Affects Intestinal Epithelial Cell Survival and Growth.PLoS One. 2020 Mar 17;15(3):e0230791. doi: 10.1371/journal.pone.0230791. eCollection 2020. PLoS One. 2020. PMID: 32182284 Free PMC article. No abstract available.
-
Deciphering the microbiome shift during fermentation of medicinal plants.Sci Rep. 2019 Sep 17;9(1):13461. doi: 10.1038/s41598-019-49799-2. Sci Rep. 2019. PMID: 31530872 Free PMC article.
-
Diets, functional foods, and nutraceuticals as alternative therapies for inflammatory bowel disease: Present status and future trends.World J Gastroenterol. 2018 Jul 7;24(25):2673-2685. doi: 10.3748/wjg.v24.i25.2673. World J Gastroenterol. 2018. PMID: 29991873 Free PMC article. Review.
-
Probiotic reduces bacterial translocation in type 2 diabetes mellitus: A randomised controlled study.Sci Rep. 2017 Sep 21;7(1):12115. doi: 10.1038/s41598-017-12535-9. Sci Rep. 2017. PMID: 28935921 Free PMC article. Clinical Trial.
-
Lactobacillus casei Exerts Anti-Proliferative Effects Accompanied by Apoptotic Cell Death and Up-Regulation of TRAIL in Colon Carcinoma Cells.PLoS One. 2016 Feb 5;11(2):e0147960. doi: 10.1371/journal.pone.0147960. eCollection 2016. PLoS One. 2016. PMID: 26849051 Free PMC article.
References
-
- Macpherson AJ, Harris NL (2004) Interactions between commensal intestinal bacteria and the immune system. Nat Rev Immunol 4: 478–485. - PubMed
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources