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. 2013 Jul;36(1):115-24.
doi: 10.1016/j.etap.2013.03.012. Epub 2013 Mar 30.

Combination treatment with platycodin D and osthole inhibits cell proliferation and invasion in mammary carcinoma cell lines

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Combination treatment with platycodin D and osthole inhibits cell proliferation and invasion in mammary carcinoma cell lines

Yiyi Ye et al. Environ Toxicol Pharmacol. 2013 Jul.

Abstract

In this study, two invasive mammary carcinoma cells (MDA-MB-231 and 4T1) were utilized to evaluate the inhibitory activities of platycodin D, osthole, and the two in combination. The anti-proliferative effect was tested using the MTT and BrdU assay, and the combination of 15μM osthole and 75μM platycodin D was used for subsequent analyses. The anti-invasive effect was evaluated by the transwell assay. The results showed that the combination treatment reduced both cell proliferation and invasion. Western blot and real-time PCR revealed that the platycodin D-osthole combination significantly decreased TβRII, Smad2, Smad3 and Smad4 gene or protein expressions, as well as effectively blocked TGF-β-induced phosphorylation of Smad2 and Smad3. Thus, this study demonstrates that the anti-cancer effects of the platycodin D-osthole combination in breast cancer cells involve proliferation inhibition and invasion blockade, both of which may be mediated by perturbations in the TGF-β/Smads pathway.

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