Towards tailored assays for cell-based approaches to toxicity testing
- PMID: 23138507
- DOI: 10.14573/altex.2012.4.359
Towards tailored assays for cell-based approaches to toxicity testing
Abstract
The call for a new toxicology is mainly a call for cellular approaches and their computational integration. This article reflects on cell models, which are necessary to facilitate the transition. A mechanistic perspective has prompted the characterization of toxicity pathways and toxicity networks in order to develop robust cell-based assays for toxicity testing. Differing use scenarios for cell systems require higher degrees of sophistication, e.g., human-on-a-chip approaches are based on complex organotypic cultures to approximate the repertoire of human physiological reactions and high-throughput tests require simplicity and robustness. The new paradigm emerging under the branding of Toxicology for the 21(st) Century needs complex models for pathway of toxicity identification and simpler assays for testing the perturbation of any given pathway. With increasing knowledge about underlying mechanisms, the needs for complexity and test specificity will change. Selective cell-based assays are desirable, especially for the detection of novel toxicants and biothreats. Examples from endocrine disruption, pyrogenicity, and especially shellfish toxin testing are used to illustrate such developments.
Similar articles
-
Three-dimensional cell cultures in toxicology.Biotechnol Genet Eng Rev. 2010;26:117-38. doi: 10.5661/bger-26-117. Biotechnol Genet Eng Rev. 2010. PMID: 21415878 Review.
-
ECVAM and new technologies for toxicity testing.Adv Exp Med Biol. 2012;745:154-80. doi: 10.1007/978-1-4614-3055-1_10. Adv Exp Med Biol. 2012. PMID: 22437818 Review.
-
A critical appraisal of the process of regulatory implementation of novel in vivo and in vitro methods for chemical hazard and risk assessment.Crit Rev Toxicol. 2014 Nov;44(10):876-94. doi: 10.3109/10408444.2014.940445. Epub 2014 Jul 24. Crit Rev Toxicol. 2014. PMID: 25058877 Review.
-
Evidence-Based Toxicology Collaboration (EBTC). Editorial.ALTEX. 2013;30(1):1. ALTEX. 2013. PMID: 23451363 No abstract available.
-
SEURAT: Safety Evaluation Ultimately Replacing Animal Testing--recommendations for future research in the field of predictive toxicology.Arch Toxicol. 2015 Jan;89(1):15-23. doi: 10.1007/s00204-014-1421-5. Epub 2014 Nov 30. Arch Toxicol. 2015. PMID: 25433540
Cited by
-
Use of Physiologically Based Kinetic Modeling-Facilitated Reverse Dosimetry to Predict In Vivo Acute Toxicity of Tetrodotoxin in Rodents.Toxicol Sci. 2022 Apr 26;187(1):127-138. doi: 10.1093/toxsci/kfac022. Toxicol Sci. 2022. PMID: 35218365 Free PMC article.
-
Comparison of methods used for evaluation of mutagenicity/genotoxicity of model chemicals - parabens.Physiol Res. 2020 Dec 31;69(Suppl 4):S661-S679. doi: 10.33549/physiolres.934615. Physiol Res. 2020. PMID: 33646007 Free PMC article. Review.
-
Organotypic Models to Study Human Glioblastoma: Studying the Beast in Its Ecosystem.iScience. 2020 Oct 1;23(10):101633. doi: 10.1016/j.isci.2020.101633. eCollection 2020 Oct 23. iScience. 2020. PMID: 33103073 Free PMC article. Review.
-
Revisiting the Neuroblastoma Cell-Based Assay (CBA-N2a) for the Improved Detection of Marine Toxins Active on Voltage Gated Sodium Channels (VGSCs).Toxins (Basel). 2020 Apr 27;12(5):281. doi: 10.3390/toxins12050281. Toxins (Basel). 2020. PMID: 32349302 Free PMC article.
-
Perspectives on In Vitro to In Vivo Extrapolations.Appl In Vitro Toxicol. 2018 Dec 1;4(4):305-316. doi: 10.1089/aivt.2016.0026. Epub 2018 Dec 8. Appl In Vitro Toxicol. 2018. PMID: 31890748 Free PMC article. Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous
