Action of bleomycin is affected by bleomycin hydrolase but not by caveolin-1
- PMID: 23076812
- DOI: 10.3892/ijo.2012.1668
Action of bleomycin is affected by bleomycin hydrolase but not by caveolin-1
Abstract
Bleomycin is a first-line clinically used antitumor antibiotic for effective treatment of certain types of cancer in combination with other antitumor agents. Its action is affected by bleomycin hydrolase (BLH), DNA repair enzymes, membrane transport proteins and other cellular factors. To clarify whether BLH confers the resistance to bleomycin in tumor cells, it is necessary to further investigate the roles of BLH and its combination with other factors such as caveolin-1 in the action of bleomycin. In this study fourteen human cell lines were used for determination of bleomycin action and roles of BLH and caveolin-1. The relationship between action of bleomycin and cellular amount of BLH was detected by the MTT method and western blotting in the human leukemia cell line HL-60, HeLa cervical cancer cells and HaCaT immortalized keratinocyte cells. The sensitivity to bleomycin was increased in HeLa cells after knockdown of BLH mRNA by RNA interference. There is no relationship between caveolin-1 levels and action of bleomycin, although the distribution of the cell cycle was altered in the caveolin-1-knockdown HeLa cells after treatment with bleomycin. In addition, regulation of BLH and caveolin-1 expression in HeLa and HaCaT cells was observed in a concentration-dependent manner after exposure to bleomycin. In conclusion, bleomycin hydrolase is one of the biomarkers for determination of bleomycin action. Although caveolin-1 can respond to bleomycin treatment, it is unrelated to bleomycin sensitivity.
Similar articles
-
Cleavage of bleomycin hydrolase by caspase-3 during apoptosis.Oncol Rep. 2013 Aug;30(2):939-44. doi: 10.3892/or.2013.2484. Epub 2013 May 23. Oncol Rep. 2013. PMID: 23708668
-
Downregulation of caveolin-1 affects bleomycin-induced growth arrest and cellular senescence in A549 cells.Int J Biochem Cell Biol. 2007;39(10):1964-74. doi: 10.1016/j.biocel.2007.05.018. Epub 2007 Jun 3. Int J Biochem Cell Biol. 2007. PMID: 17662641
-
Bleomycin induces caveolin-1 and -2 expression in epithelial lung cancer A549 cells.Anticancer Res. 2007 May-Jun;27(3A):1343-51. Anticancer Res. 2007. PMID: 17593629
-
Bleomycin.Cancer Chemother Biol Response Modif. 1999;18:39-45. Cancer Chemother Biol Response Modif. 1999. PMID: 10800476 Review. No abstract available.
-
Biochemical and cellular determinants of bleomycin cytotoxicity.Cancer Surv. 1986;5(1):81-91. Cancer Surv. 1986. PMID: 2439200 Review.
Cited by
-
Resistance to bleomycin in cancer cell lines is characterized by prolonged doubling time, reduced DNA damage and evasion of G2/M arrest and apoptosis.PLoS One. 2013 Dec 4;8(12):e82363. doi: 10.1371/journal.pone.0082363. eCollection 2013. PLoS One. 2013. PMID: 24349265 Free PMC article.
-
Molecular Characterization of pBOq-IncQ and pBOq-95LK Plasmids of Escherichia coli BOq 01, a New Isolated Strain from Poultry Farming, Involved in Antibiotic Resistance.Microorganisms. 2022 Jul 26;10(8):1509. doi: 10.3390/microorganisms10081509. Microorganisms. 2022. PMID: 35893567 Free PMC article.
-
The Interaction of the Metallo-Glycopeptide Anti-Tumour Drug Bleomycin with DNA.Int J Mol Sci. 2018 May 4;19(5):1372. doi: 10.3390/ijms19051372. Int J Mol Sci. 2018. PMID: 29734689 Free PMC article. Review.
-
Decrease of ABCB1 protein expression and increase of G1 phase arrest induced by oleanolic acid in human multidrug-resistant cancer cells.Exp Ther Med. 2021 Jul;22(1):735. doi: 10.3892/etm.2021.10167. Epub 2021 May 9. Exp Ther Med. 2021. PMID: 34055052 Free PMC article.
-
Battling Chemoresistance in Cancer: Root Causes and Strategies to Uproot Them.Int J Mol Sci. 2021 Aug 31;22(17):9451. doi: 10.3390/ijms22179451. Int J Mol Sci. 2021. PMID: 34502361 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
