Early B cell factor 1 regulates B cell gene networks by activation, repression, and transcription- independent poising of chromatin
- PMID: 20451411
- DOI: 10.1016/j.immuni.2010.04.013
Early B cell factor 1 regulates B cell gene networks by activation, repression, and transcription- independent poising of chromatin
Abstract
The transcription factor early B cell factor-1 (Ebf1) is a key determinant of B lineage specification and differentiation. To gain insight into the molecular basis of Ebf1 function in early-stage B cells, we combined a genome-wide ChIP sequencing analysis with gain- and loss-of-function transcriptome analyses. Among 565 genes that are occupied and transcriptionally regulated by Ebf1, we identified large sets involved in (pre)-B cell receptor and Akt signaling, cell adhesion, and migration. Interestingly, a third of previously described Pax5 targets was found to be occupied by Ebf1. In addition to Ebf1-activated and -repressed genes, we identified targets at which Ebf1 induces chromatin changes that poise the genes for expression at subsequent stages of differentiation. Poised chromatin states on specific targets could also be established by Ebf1 expression in T cells but not in NIH 3T3 cells, suggesting that Ebf1 acts as a "pioneer" factor in a hematopoietic chromatin context.
Copyright 2010 Elsevier Inc. All rights reserved.
Similar articles
-
Combined heterozygous loss of Ebf1 and Pax5 allows for T-lineage conversion of B cell progenitors.J Exp Med. 2015 Jun 29;212(7):1109-23. doi: 10.1084/jem.20132100. Epub 2015 Jun 8. J Exp Med. 2015. PMID: 26056231 Free PMC article.
-
Transcription factor Pax5 activates the chromatin of key genes involved in B cell signaling, adhesion, migration, and immune function.Immunity. 2007 Jul;27(1):49-63. doi: 10.1016/j.immuni.2007.05.019. Epub 2007 Jul 19. Immunity. 2007. PMID: 17658281
-
Pioneering Activity of the C-Terminal Domain of EBF1 Shapes the Chromatin Landscape for B Cell Programming.Immunity. 2016 Mar 15;44(3):527-541. doi: 10.1016/j.immuni.2016.02.021. Immunity. 2016. PMID: 26982363
-
Defining B Cell Chromatin: Lessons from EBF1.Trends Genet. 2018 Apr;34(4):257-269. doi: 10.1016/j.tig.2017.12.014. Epub 2018 Jan 11. Trends Genet. 2018. PMID: 29336845 Review.
-
B lymphocyte lineage specification, commitment and epigenetic control of transcription by early B cell factor 1.Curr Top Microbiol Immunol. 2012;356:17-38. doi: 10.1007/82_2011_139. Curr Top Microbiol Immunol. 2012. PMID: 21735360 Free PMC article. Review.
Cited by
-
Gene set control analysis predicts hematopoietic control mechanisms from genome-wide transcription factor binding data.Exp Hematol. 2013 Apr;41(4):354-66.e14. doi: 10.1016/j.exphem.2012.11.008. Epub 2012 Dec 4. Exp Hematol. 2013. PMID: 23220237 Free PMC article.
-
3D genome organization during lymphocyte development and activation.Brief Funct Genomics. 2020 Mar 23;19(2):71-82. doi: 10.1093/bfgp/elz030. Brief Funct Genomics. 2020. PMID: 31819944 Free PMC article.
-
Early B cell factor 4 modulates FAS-mediated apoptosis and promotes cytotoxic function in human immune cells.Proc Natl Acad Sci U S A. 2022 Aug 16;119(33):e2208522119. doi: 10.1073/pnas.2208522119. Epub 2022 Aug 8. Proc Natl Acad Sci U S A. 2022. PMID: 35939714 Free PMC article.
-
The transcription factor early B-cell factor 1 regulates bone formation in an osteoblast-nonautonomous manner.FEBS Lett. 2013 Mar 18;587(6):711-6. doi: 10.1016/j.febslet.2013.01.049. Epub 2013 Feb 5. FEBS Lett. 2013. PMID: 23395800 Free PMC article.
-
Expression of essential B cell development genes in horses with common variable immunodeficiency.Mol Immunol. 2012 Jun;51(2):169-76. doi: 10.1016/j.molimm.2012.03.018. Epub 2012 Mar 30. Mol Immunol. 2012. PMID: 22464097 Free PMC article.
Publication types
MeSH terms
Substances
Associated data
- Actions
- Actions
- Actions
- Actions
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
