RNA-Seq: a revolutionary tool for transcriptomics
- PMID: 19015660
- PMCID: PMC2949280
- DOI: 10.1038/nrg2484
RNA-Seq: a revolutionary tool for transcriptomics
Abstract
RNA-Seq is a recently developed approach to transcriptome profiling that uses deep-sequencing technologies. Studies using this method have already altered our view of the extent and complexity of eukaryotic transcriptomes. RNA-Seq also provides a far more precise measurement of levels of transcripts and their isoforms than other methods. This article describes the RNA-Seq approach, the challenges associated with its application, and the advances made so far in characterizing several eukaryote transcriptomes.
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