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Comparative Study
. 2007 Jul;73(13):4152-61.
doi: 10.1128/AEM.00222-07. Epub 2007 May 4.

Molecular identification and genetic analysis of Norovirus genogroups I and II in water environments: comparative analysis of different reverse transcription-PCR assays

G La Rosa  1 , S Fontana, A Di Grazia, M Iaconelli, M Pourshaban, M Muscillo
Affiliations
Comparative Study

Molecular identification and genetic analysis of Norovirus genogroups I and II in water environments: comparative analysis of different reverse transcription-PCR assays

G La Rosa et al. Appl Environ Microbiol. 2007 Jul.

Erratum in

  • Appl Environ Microbiol. 2007 Oct;73(19):6329

Abstract

Noroviruses have received increased attention in recent years because their role as etiologic agents in acute gastroenteritis outbreaks is now clearly established. Our inability to grow them in cell culture and the lack of an animal model hinder the characterization of these viruses. More recently, molecular approaches have been used to study the genetic relationships that exist among them. In the present study, environmental samples from seawater, estuarine water, and effluents of sewage treatment plants were analyzed in order to evaluate the role of environmental surface contamination as a possible vehicle for transmission of norovirus genogroups I and II. Novel broad-range reverse transcription-PCR/nested assays targeting the region coding for the RNA-dependent RNA polymerase were developed, amplifying fragments of 516 bp and 687 bp in the nested reactions for genogroups II and I, respectively. The assays were evaluated and compared against widely used published assays. The newly designed assays provide long regions for high-confidence BLAST searches in public databases and therefore are useful diagnostic tools for molecular diagnosis and typing of human noroviruses in clinical and environmental samples, as well as for the study of molecular epidemiology and the evolution of these viruses.

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Figures

FIG. 1.
FIG. 1.
Sample collection sites along 120 km of the Latium coast. Symbols: circles, seawater samples; squares, sewage samples.
FIG. 2.
FIG. 2.
Phylogenetic tree constructed in the RdRp region (56). The type of sample from which the sequence was obtained is listed in parentheses (C, clinical samples; S, seawater, SW, sewage; R, reference).
FIG. 3.
FIG. 3.
Phylogenetic tree constructed in the RdRp region for NoV (this study). The abbreviations in parentheses are the same as those listed in the legend to Fig. 2.
See this image and copyright information in PMC

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