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. 2006 Apr;47(4):804-14.
doi: 10.1194/jlr.M500506-JLR200. Epub 2006 Jan 28.

LC-MS-based method for the qualitative and quantitative analysis of complex lipid mixtures

Ulf Sommer  1 , Haya Herscovitz, Francine K Welty, Catherine E Costello
Affiliations
Free article

LC-MS-based method for the qualitative and quantitative analysis of complex lipid mixtures

Ulf Sommer et al. J Lipid Res. 2006 Apr.
Free article

Abstract

A simple and robust LC-MS-based methodology for the investigation of lipid mixtures is described, and its application to the analysis of human lipoprotein-associated lipids is demonstrated. After an optional initial fractionation on Silica 60, normal-phase HPLC-MS on a YMC PVA-Sil column is used first for class separation, followed by reversed-phase LC-MS or LC-tandem mass spectrometry using an Atlantis dC18 capillary column, and/or nanospray MS, to fully characterize the individual lipids. The methodology is applied here for the analysis of human apolipoprotein B-associated lipids. This approach allows for the determination of even low percentages of lipids of each molecular species and showed clear differences between lipids associated with apolipoprotein B-100-LDL isolated from a normal individual and those associated with a truncated version, apolipoprotein B-67-containing lipoproteins, isolated from a homozygote patient with familial hypobetalipoproteinemia. The methods described should be easily adaptable to most modern MS instrumentation.

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